RNA Polymerase Selection Chart

T7 and SP6 RNA polymerases are DNA dependent RNA polymerases that produce DNA templated RNA transcripts. T7 and SP6 exhibit high specificity for their respective promoters. Both T7 and SP6 can be used for the in vitro synthesis of RNA for a wide variety of applications, including transfection, translation, structural studies and radioactive and non-isotopic probe generation. E. coli Poly(A) Polymerase and Poly(U) Polymerase generate untemplated homoribopolymeric tails on RNA 3´-ends. Both E. coli Poly(A) Polymerase and Poly(U) Polymerase can be used for RNA tailing prior to reverse transcription or for direct labeling.

ProductNEB #ApplicationsSize
T3 RNA PolymeraseM0378S
  • Radiolabeled RNA probes
  • Non-isotopic RNA labeling
  • Preparation of RNA vaccines
  • Guide RNA for gene targeting
  • mRNA for in vitro translation and micro injection
  • RNA structure, processing and catalysis studies
  • RNA amplification
  • Anti-sense RNA for gene expression experiment
5,000 units
T7 RNA PolymeraseM0251S
M0251L
  • Radiolabeled RNA probes
  • Non-isotopic RNA labeling
  • Preparation of RNA vaccines
  • Guide RNA for gene targeting
  • mRNA for in vitro translation and micro injection 
  • RNA structure, processing and catalysis studies
  • RNA amplification
  • Anti-sense RNA for gene expression experiment 
5,000 units
25,000 units
SP6 RNA PolymeraseM0207S
M0207L
  • Radiolabeled RNA probe preparation
  • Non-isotopic RNA labeling
  • Preparation for RNA vaccines
  • Guide RNA for gene targeting
  • mRNA for in vitro translation and micro injection
  • RNA structure, processing and catalysis studies
  • RNA amplification
  • Anti-sense RNA for gene expression experiment
2,000 units
10,000 units
E. coli Poly(A) PolymeraseM0276S
M0276L
  • Labeling of RNA with ATP or cordycepin
  • Poly(A) tailing of RNA for cloning or affinity purification
  • Enhances translation of RNA transferred into eukaryotic cells.
100 units
500 units
Poly(U) PolymeraseM0337S
  • Labeling of RNA with UTP
  • Poly(U) and Poly(A) tailing of RNA for cloning
  • Studying effects of poly(U) tailing on stability and translation of RNA transferred into eukaryotic cells
  • Poly (A) tailing of 2' O-Me modified 3' ends
60 units
E. coli RNA Polymerase, Core EnzymeM0550S
  • RNA synthesis from E. coli promoter
  • Transcription initiation studies
  • In vitro translation with PURExpress
100 units
E. coli RNA Polymerase, HoloenzymeM0551S
  • RNA synthesis from E. coli promoter 
  • Transcription initiation studies
  • In vitro translation with PURExpress
50 units