Protease Selection Chart

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  • NEB offers several proteases with different recognition sites.

    Product NEB # Recognition Site
    Factor Xa Protease P8010 Ile-Glu/Asp-Gly-Arg
    Enterokinase (enteropeptidase), light chain P8070 Asp-Asp-Asp-Asp-Lys
    Genenase™ I P8075 His-Tyr or Tyr-His
    Furin P8077 Arg-X-X-Arg
    Proteinase K P8102 non-specific, subtilisin-related serine protease
    Modified Trypsin (TPCK-treated) P8101 Lys/Arg-X-X-X
    Endoproteinase GluC P8100 Glu-X-X-X or Asp-X-X-X
    Endoproteinase AspN P8104 X-X-X-Asp