All recognition sequences are written 5' to 3' using the single letter code nomenclature. Numbers in parentheses indicate the point of cleavage.
Example, GGTCTC(1/5) indicates cleavage at:
The Q5® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours.
The Gibson Assembly™ Cloning Kit is a robust method for assembling 1-2 DNA fragments, with a cloning vector, and ensuring high transformation efficiencies - all in a little less than 2 hours.