Enzyme Finder - Sequence > 6 Bases

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  • Enzyme Sequence Cut Site Overhang Properties (NEB Enzymes Only)
    Nb.BbvCI CCTCAGC
    C C T C A G C
    G G A G T / C G
    nick     
    BbvCI CCTCAGC
    C C / T C A G C
    G G A G T / C G
    5´ - TCA      
    Nt.BbvCI CCTCAGC
    C C / T C A G C
    G G A G T C G
    nick
        
    AscI GGCGCGCC
    G G / C G C G C C
    C C G C G C / G G
    5´ - CGCG       
    AscI RE-Mix® GGCGCGCC
    G G / C G C G C C
    C C G C G C / G G
    5´ - CGCG     
    AsiSI GCGATCGC
    G C G A T / C G C
    C G C / T A G C G

    AT - 3´       
    FseI GGCCGGCC
    G G C C G G / C C
    C C / G G C C G G
    CCGG - 3´        
    NotI GCGGCCGC
    G C / G G C C G C
    C G C C G G / C G
    5´ - GGCC       
    NotI-HF™ RE-Mix® GCGGCCGC
    G C / G G C C G C
    C G C C G G / C G
    5´ - GGCC     
    NotI-HF™ GCGGCCGC
    G C G G C C G C
    C G C C G G C G
    5´ - GGCC       
    PacI TTAATTAA
    T T A A T / T A A
    A A T / T A A T T
    AT - 3´        
    PacI RE-Mix® TTAATTAA
    T T A A T / T A A
    A A T / T A A T T
    AT - 3´     
    PmeI GTTTAAAC
    G T T T / A A A C
    C A A A / T T T G
    blunt        
    SbfI CCTGCAGG
    C C T G C A / G G
    G G / A C G T C C
    TGCA - 3´      
    SbfI-HF™ CCTGCAGG
    C C T G C A G G
    G G A C G T C C
    TGCA - 3´      
    SgrAI CRCCGGYG
    C R / C C G G Y G
    G Y G G C C / R C
    5´ - CCGG     
    SwaI ATTTAAAT
    A T T T / A A A T
    T A A A / T T T A
    blunt      
    Nt.BspQI GCTCTTC
    G C T C T T C N /
    C G A G A A G N
    nick      
    BspQI GCTCTTC
    G C T C T T C
    C G A G A A G
    5´ - NNN      
    SapI GCTCTTC
    G C T C T T C
    C G A G A A G
    5´ - NNN     
    SfiI GGCCNNNNNGGCC
    G G C C N N N N / N G G C C
    C C G G N / N N N N C C G G
    NNN - 3´        
    CspCI CAANNNNNGTGG
    C A A N N N N N G T G G
    G T T N N N N N C A C C
    NN - 3´       
    AbsI CCTCGAGG
    C C / T C G A G G
    G G A G C T / C C
    5´ - TCGA
    CciNI GCGGCCGC
    G C / G G C C G C
    C G C C G G / C G
    5´ - GGCC
    FspAI RTGCGCAY
    R T / G C / G C A Y
    Y A C G / C / G T R
    blunt
    MauBI CGCGCGCG
    C G / C G C G C G
    G C G C G C / G C
    5´ - CGCG
    MreI CGCCGGCG
    C G / C C G G C G
    G C G G C C / G C
    5´ - CCGG
    MssI GTTTAAAC
    G T T T / A A A C
    C A A A / T T T G
    blunt
    PalAI GGCGCGCC
    G G / C G / C G C C
    C C G C / G C / G G
    5´ - CGCG
    RgaI GCGATCGC
    G C G A T / C G C
    C G C / T A G C G
    AT - 3´
    RigI GGCCGGCC
    G G C C G / G / C C
    C C / G G C C G G
    CCGG - 3´
    SdaI CCTGCAGG
    C C T G C A / G G
    G G / A C G T C C
    TGCA - 3´
    SfaAI GCGATCGC
    G C G A T / C G C
    C G C / T A G C G
    AT - 3´
    SgfI GCGATCGC
    G C G A T / C G C
    C G C / T A G C G
    AT - 3´
    SgrDI CGTCGACG
    C G T C G A C G N N
    N N G C A G C T G C
    5´ - TCGA
    SgsI GGCGCGCC
    G G / C G C G C C
    C C G C G C / G G
    5´ - CGCG
    SmiI ATTTAAAT
    A T T T / A A A T
    T A A A / T T T A
    blunt
    SrfI GCCCGGGC
    G C C C / G G G C
    C G G G / C C C G
    blunt
    Sse232I CGCCGGCG
    C G / C C G G C G
    G C G G C C / G C
    5´ - CCGG
    Sse8387I CCTGCAGG
    C C / T G C A / G G
    G G / A C G / T C C
    TGCA - 3´
    LguI GCTCTTC
    G C T C T T C
    C G A G A A G
    5´ - NNN
    PciSI GCTCTTC
    G C T C T T C N / N N N
    C G A G A A G N N N N /
    5´ - NNN
    AarI CACCTGC
    C A C C T G C
    G T G G A C G
    5´ - NNNN
    AjuI GAANNNNNNNTTGG
    G A A N N N N N N N T T G G
    C T T N N N N N N N A A C C
    NNNNN - 3´
    AloI GAACNNNNNNTCC
    G A A C N N N N N N T C C
    C T T G N N N N N N A G G
    NNNNN - 3´
    BarI GAAGNNNNNNTAC
    G A A G N N N N N N T A C
    C T T C N N N N N N A T G
    NNNNN - 3´
    PpiI GAACNNNNNCTC
    G A A C N N N N N C T C
    C T T G N N N N N G A G
    NNNNN - 3´
    PsrI GAACNNNNNNTAC
    G A A C N N N N N N T A C
    C T T G N N N N N N A T G
    NNNNN - 3´