New England Biolabs

E. coli Strains Supplied by NEB

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  • The following E. coli strains are available upon request from New England Biolabs at no charge with an order or for the cost of shipping if ordered separately. They are supplied in vials containing approximately 200 µl of a 50% glycerol suspension. The strains are not competent.

    The table summarizes relevant characteristics and recommended applications for each strain. Strain genotypes and brief descriptions are listed below. Genotypes of E. coli strains sold by NEB as ready-to-use competent cells can be found in Competent E. coli Strains from NEB.

      Library
    Construc
    -tion1
    Plasmid
    Prepara
    -tion2
    Cloning/
    Sub-
    cloning
    Dam-/
    Dcm-
    Single-
    stranded
    Phage3
    Blue/
    White
    Screening
    RecA- Protease-
    deficient4
    Laclq
    (for P lac
    regulation)
    Kunkel
    Mutage-
    nesis
    Drug
    Resistance5
    ER1793 + + str
    ER1821 + + + none
    ER2267 + + + + + + + kan
    ER2738 + + + + tet
    ER2925(6) + + cam 
    str
    JM101 + + + none
    JM109 + + + + + + nal
    NM522 + + + + none
    ER2507 + kan
    str
    TB1 + + str
    CAG597 + tet
    str
    CAG626 + str
    CAG629 + tet 
    str
    PR1031 + tet
    ER2508 + kan
    str
    tet
    KS1000 + + + kan
    nal
    tet
    UT5600 + str
    CJ236 + + cam

    Notes

    1. Restriction-deficient strain.
    2. Strain has a mutation in the endA gene which eliminates the major nonspecific endonuclease.
    3. Strain contains F´.
    4. See strain description for details.
    5. cam = chloramphenicol; kan = kanamycin; nal = nalidixic acid; str = streptomycin; tet = tetracycline
    6. Also sold as ready-to-use competent cells (NEB #C2925H).