New England Biolabs: Reagents for the Life Sciences Industry
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  • DNA Ligase Selection Chart

    While more than one ligase may work for your application, the following selection chart presents our recommendations for optimal performance.


    Blunt/TA
    Ligase
    Master Mix
    Instant Sticky-end Master Mix ElectroLigase® T4 DNA Ligase Quick
    Ligation™
    Kit
    T3 DNA
    Ligase
    T7 DNA
    Ligase
    E. coli DNA
    Ligase
    Taq DNA
    Ligase
    9°N™ DNA
    Ligase
    NEBNext Quick Ligation™ Module SplintR® Ligase
    DNA APPLICATIONS
    Ligation of sticky ends ★★ ★★★ ★★ ★★ ★★★ ★★ ★★
     
    Ligation of blunt ends ★★★ ★★ ★★ ★★★ ★★




     
    T/A cloning ★★★ ★★ ★★ ★★



     
    Electroporation

    ★★★ ★★






     
    Ligation of sticky ends only





    ★★★



     
    Repair of nicks in dsDNA ★★★ ★★★ ★★★ ★★★ ★★★ ★★★ ★★★ ★★★ ★★★ ★★★
    High-complexity library cloning ★★ ★★ ★★ ★★★ ★★





     
    Ligase Detection Reaction & Ligase Chain Reaction







    ★★ ★★★
     
    Ligation of adjacent ssDNAs on an RNA splint







       
     ★★★
    NGS APPLICATIONS
    NGS Library Prep dsDNA-dsDNA (ligation)






     
    FEATURES
    Salt tolerance
    (>2X that for T4 DNA Ligase)










     
    Ligation in 15 min. or less


    Master Mix Formulation







     
    Thermostable








     
    Recombinant
    KEY
    ★★★ Optimal, recommended ligase for selected application
    ★★ Works well for selected application
    Will perform selected application, but is not recommended
    Please consult the specific NGS protocol to determine the optimal enzyme for your needs

    ElectroLigase™, Quick Ligation™ and 9°N™ are trademarks of New England Biolabs, Inc.