New England Biolabs: Reagents for the Life Sciences Industry
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  • DNA Ligase Selection Chart

    While more than one ligase may work for your application, the following selection chart presents our recommendations for optimal performance.


    Blunt/TA
    Ligase
    Master Mix
    Instant Sticky-end Master Mix ElectroLigase® T4 DNA Ligase Quick
    Ligation™
    Kit
    T3 DNA
    Ligase
    T7 DNA
    Ligase
     HiFi Taq DNA Ligase
    Taq DNA
    Ligase
    9°N™ DNA
    Ligase
    NEBNext Quick Ligation™ Module SplintR® Ligase  E.coli DNA Ligase
    DNA APPLICATIONS
    Ligation of sticky ends ★★ ★★★ ★★ ★★ ★★★ ★★ ★★
       ★
    Ligation of blunt ends ★★★ ★★ ★★ ★★★ ★★
     


       
    T/A cloning ★★★ ★★ ★★ ★★  


       
    Electroporation

    ★★★ ★★


     


       
    Ligation of sticky ends only





    ★★★  


       
    Repair of nicks in dsDNA ★★ ★★ ★★ ★★★ ★★ ★★ ★★  ★★ ★★ ★★
    ★★
     ★★
    High-complexity library cloning ★★ ★★ ★★ ★★★ ★★

     


       
    Adaptor Ligation    ▲
    ★★★ ★★ ★★ ★  ★★  ★          ▲    
    Ligation-Dependent DNA Sequence & SNP Detection (LCR, LDR & related methods)







     ★★★ ★★ ★★
       
    Ligation-Dependent RNA Sequence & SNP Detection







         
     ★★★  
    Ligation of adjacent ssDNAs on an RNA splint
                          ★★★  
    NGS APPLICATIONS
    NGS Library Prep dsDNA-dsDNA (ligation)



     

       
    FEATURES
    Salt tolerance
    (>2X that for T4 DNA Ligase)






     


       
    Ligation in 15 min. or less

     ✓  
    Master Mix Formulation




     

       
    Thermostable






     ✓
       
    Recombinant  ✓  ✓
    KEY
    ★★★ Optimal, recommended ligase for selected application
    ★★ Works well for selected application
    Will perform selected application, but is not recommended
    Please consult the specific NGS protocol to determine the optimal enzyme for your needs

    ElectroLigase™, SplintR®, Quick Ligation™ and 9°N™ are trademarks of New England Biolabs, Inc.