New England Biolabs: Reagents for the Life Sciences Industry
New England Biolabs is pleased to announce that on July 25th we changed our domestic carrier to UPS. Should you have any questions or issues regarding this change, please contact our Customer Service team at 1-800-632-5227 or via email at customerservice@neb.com.close
  • My NEB
  • Print
  • PDF
  • DNA Ligase Selection Chart

    While more than one ligase may work for your application, the following selection chart presents our recommendations for optimal performance.


    Blunt/TA
    Ligase
    Master Mix
    Instant Sticky-end Master Mix ElectroLigase® T4 DNA Ligase Quick
    Ligation™
    Kit
    T3 DNA
    Ligase
    T7 DNA
    Ligase
    E. coli DNA
    Ligase
    Taq DNA
    Ligase
    9°N™ DNA
    Ligase
    NEBNext Quick Ligation™ Module SplintR® Ligase
    DNA APPLICATIONS
    Ligation of sticky ends ★★ ★★★ ★★ ★★ ★★★ ★★ ★★
     
    Ligation of blunt ends ★★★ ★★ ★★ ★★★ ★★




     
    T/A cloning ★★★ ★★ ★★ ★★



     
    Electroporation

    ★★★ ★★






     
    Ligation of sticky ends only





    ★★★



     
    Repair of nicks in dsDNA ★★★ ★★★ ★★★ ★★★ ★★★ ★★★ ★★★ ★★★ ★★★ ★★★
    High-complexity library cloning ★★ ★★ ★★ ★★★ ★★





     
    Ligase Detection Reaction & Ligase Chain Reaction







    ★★ ★★★
     
    Ligation of adjacent ssDNAs on an RNA splint







       
     ★★★
    NGS APPLICATIONS
    NGS Library Prep dsDNA-dsDNA (ligation)






     
    FEATURES
    Salt tolerance
    (>2X that for T4 DNA Ligase)










     
    Ligation in 15 min. or less


    Master Mix Formulation







     
    Thermostable








     
    Recombinant
    KEY
    ★★★ Optimal, recommended ligase for selected application
    ★★ Works well for selected application
    Will perform selected application, but is not recommended
    Please consult the specific NGS protocol to determine the optimal enzyme for your needs

    ElectroLigase™, SplintR®, Quick Ligation™ and 9°N™ are trademarks of New England Biolabs, Inc.