Sequences at the top of each column are written 5´ to 3´ according to convention. Open squares at the left of each row are place holders for nucleotides within a restriction endonuclease recognition sequence; arrowheads indicate the point of cleavage. Sequences of complementary strands and their cleavage sites are implied.
orange type = enzymes that recognize only one sequence
black type = enzymes that recognize multiple sequences (degenerate)
Tetra- and Hexa-Nucleotide Recognition Sequences | Penta-Nucleotide Recognition Sequences
* HF (high fidelity) versions of these enzymes are available for simplified reactions and reduced star activity, at no additional cost.
The NEB PCR Cloning Kit allows direct cloning from any amplification reaction, with no purification needed!
The Gibson Assembly™ Cloning Kit is a robust method for assembling 1-2 DNA fragments, with a cloning vector, and ensuring high transformation efficiencies - all in a little less than 2 hours.