Sequences at the top of each column are written 5´ to 3´ according to convention. Open squares at the left of each row are place holders for nucleotides within a restriction endonuclease recognition sequence; arrowheads indicate the point of cleavage. Sequences of complementary strands and their cleavage sites are implied.
orange type = enzymes that recognize only one sequence
black type = enzymes that recognize multiple sequences (degenerate)
Tetra- and Hexa-Nucleotide Recognition Sequences | Penta-Nucleotide Recognition Sequences
The NEB PCR Cloning Kit allows direct cloning from any amplification reaction, with no purification needed!
The Gibson Assembly™ Cloning Kit is a robust method for assembling 1-2 DNA fragments, with a cloning vector, and ensuring high transformation efficiencies - all in a little less than 2 hours.