New England Biolabs

Competent E. coli Strains

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  • ProductNEB #Characteristics
    Strains for Cloning and Subcloning
    NEB Turbo Competent E. coli (High Efficiency)C2984
    • Fastest growth – colonies visible after 6.5 hours
    • Plasmid preparation after 4 hours
    • Tight control/expression of toxic genes
    NEB 5-alpha Competent E. coli (High Efficiency)C2987
    • Versatile cloning strain
    • DH5α™ derivative
    NEB 5-alpha F'Iq Competent E. coli (High Efficiency)C2992
    • Toxic gene cloning
    • F´ strain with extremely high transformation efficiency
    NEB 10-beta Competent E. coli (High Efficiency)C3019
    • Large plasmid and BAC cloning
    • DH10B™ derivative
    dam-/dcm- Competent E. coliC2925
    • Dam/Dcm methyltransferase free plasmid growth
    Strains for Protein Expression
    NEB Express Competent E. coli (High Efficiency)C2523
    • Versatile non-T7 expression strain
    • Protease deficient
    NEB Express Iq Competent E. coli (High Efficiency)C3037
    • Control of IPTG induced expression from Plac, Ptac and Ptrc
    • Protease deficient
    T7 Express Competent E. coli (High Efficiency)C2566
    • Most popular T7 expression strain
    • Protease deficient
    T7 Express Iq Competent E. coli (High Efficiency)C3016
    • T7 expression
    • Protease deficient
    • Reduced basal expression
    T7 Express lysY Competent E. coli (High Efficiency)C3010
    • T7 expression
    • Protease deficient
    • Better reduction of basal expression
    T7 Express lysY/Iq Competent E. coli (High Efficiency)C3013
    • T7 expression
    • Protease deficient
    • Highest level of expression control
    T7 Express Crystal Competent E. coli (High Efficiency)C3022
    • T7 expression
    • Protease deficient
    • SeMet labeling for protein crystallography
    SHuffle® Express Competent E. coliC3028
    • Protease deficient/B strain
    • Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm
    SHuffle® T7 Express Competent E. coliC3029
    • T7 expression
    • Protease deficient/B strain
    • Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm
    SHuffle® T7 Express lysY Competent E. coliC3030
    • T7 expression
    • Protease deficient/B strain
    • Tightly controlled expression of toxic proteins
    • Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm
    SHuffle® T7 Competent E. coliC3026
    • T7 expression/K12 strain
    • Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm
    BL21 Competent E. coliC2530
    • Routine non-T7 expression
    BL21(DE3) Competent E. coliC2527
    • Routine T7 expression
    Lemo21(DE3) Competent E. coliC2528
    • Tunable T7 expression for difficult targets
    NiCo21(DE3) Competent E. coliC2529
    • Improved purity of target proteins isolated by IMAC

    There are many properties to consider when choosing a strain for your experiments. Requirements such as plasmid preparation, blue-white screening, cytoplasmic disulfide bond formation and fast colony growth necessitate specific strain choices. Please check the Strain Properties Chart for a particular experiment.

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