Cloning Plasmids and DNAs

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  • Cloning Plasmid/DNA NEB # Features Concentration MW/Size Size Price
    LITMUS 28i Vector N3528S
    • Multipurpose cloning plasmids
    • Unique MCS includes many restriction enzymes with 4-base overhangs
    • T7 promoter
    • Amp resistance
    • Blue/white selection
    500 µg/ml 1.8 x 106 Da/
    2,823 bp
    20 µg
    $88
    pBR322 Vector N3033S/L
    • Commonly used cloning vectors
    • Tet, Amp resistance
    1,000 µg/ml
    2.83 x 106 DA/
    4,361 bp
    50/250 µg
    $65/$260
    pUC19 Vector N3041S/L 1,000 µg/ml
    1.75 x 106 Da/
    2,686 bp
    50/250 µg
    $65/$260
    M13mp18 RF I DNA N4018S
    • Phage vectors derived from bacteriophage M13
    • DNA, covalently closed circular
    • 13 Unique RE sites with β-gal gene
    • Blue-white selection
    100 µg/ml
    7,249 bp 10 µg
    $74
    M13mp18 Single-stranded DNA N4040S 250 µg/ml
    7,249 bases 10 µg
    $33
    Lambda DNA N3011S/L
    • Commonly used DNA substrate
    500 µg/ml
    31.5 x 106 Da/
    48,502 bp
    250/1,250 µg
    $62/$248
    Lambda DNA (N6-methyladenine-free) N3013S/L 500 µg/ml
    31.5 x 106 Da/
    48,502 bp
    250/1,250 µg
    $62/$248
    ΦX174 RF I DNA
    N3021S/L
    • Commonly used DNA substrate
    • Covalently closed circular form of X174
    1,000 µg/ml
    3.5 x 106 Da/
    5,386 bp
    30/150 µg
    $65/$260
    ΦX174 Virion DNA
    N3023S/L
    • Single-stranded viral DNA
    1,000 µg/ml
    1.7 x 106 Da/
    5,386 bases
    50/250 µg
    $71/$284