The nucleotide sequence files available below are those used to the produce the plasmid vector, viral and bacteriophage maps contained in New England Biolabs Catalog as well as the tables containing the locations of sites. Maps and location of sites are PDF files. Sequence files are in FASTA or/and GenBank format. Detailed frequencies with which restriction endonuclease sites occur in commonly used DNA molecules can be found on Frequencies of Restriction Sites.
The NEB PCR Cloning Kit allows direct cloning from any amplification reaction, with no purification needed!