RNA Reagents
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  • RNA Synthesis Kits

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    Learn about RNA Synthesis

    HiScribe™ T7 ARCA mRNA Kit (with tailing)

    The HiScribe T7 ARCA mRNA Kit (with tailing) (NEB #E2060) is designed for quick production of ARCA capped and poly(A) tailed mRNA in vitro. Capped mRNAs are synthesized by co-transcriptional incorporation of Anti-Reverse Cap Analog (ARCA ,NEB #S1411) using T7 RNA Polymerase. The transcription reaction can be set up easily by combining the ARCA/NTP mix, T7 RNA Polymerase Mix and a suitable DNA template. The kit also allows for partial incorporation of 5mCTP, Pseudo-UTP and other modified nucleotides into mRNA. After a brief DNase I treatment to remove the template DNA, capped mRNA is poly(A) tailed with Poly(A) Polymerase. mRNAs synthesized with the kit can be used for cell transfection, microinjection, in vitro translation and RNA vaccines.

    ARCA is incorporated into mRNA exclusively in the correct orientation, generating capped mRNA that is more efficiently translated. Standard cap analogs can be incorporated in either direction resulting in only 50% of capped mRNA that is functional in protein translation.

    Advantages of the HiScribe T7 ARCA mRNA Kit (with tailing)

    • High yield – Up to 30 μg of capped & tailed mRNA per reaction
    • Streamlined workflow – mRNA capping, DNA removal, mRNA tailing & purification in 2 hrs
    • Flexibility – Enables partial incorporation of 5mCTP, Pseudo-UTP and other modified CTPs and UTPs
    • High quality mRNAs – Ultra high quality components ensure mRNA integrity
    • ARCA-based – Capping in correct orientation ensures high translation efficiency
    • Complete system – Template removal and mRNA purification reagents included
    • More reactions – Twice the number of reactions as compared to competitors’ kits

    HiScribe T7 ARCA mRNA Kit

    By using a DNA template encoding a poly(A) tail, the HiScribe T7 ARCA mRNA Kit (NEB #E2065) can be used to synthesize capped and tailed mRNAs. The cap structure is added to the mRNA by co-transcriptional incorporation of Anti-Reverse Cap Analog (ARCA) (NEB #S1411) using T7 RNA Polymerase. Poly(A) tail is incorporated during the transcription reaction. The kit also includes DNase I and LiCl for DNA template removal and quick mRNA purification.

    Additionally, the kit is capable of partial incorporation of modified UTP and CTP (up to 50% each) without affecting the mRNA yield significantly. By using a DNA template encoding a poly(A) tail, capped and tailed modified mRNA can be synthesized in a single reaction in 30 minutes. mRNAs synthesized with the kit can be used for cell transfection, microinjection, in vitro translation and RNA vaccines.

    ARCA is incorporated into mRNA exclusively in the correct orientation, generating capped mRNA that is more efficiently translated. Standard cap analogs can be incorporated in either direction resulting in only 50% of capped mRNA that is functional in protein translation.

    Advantages of the HiScribe T7 ARCA mRNA Kit

    • High yield – Up to 30 μg of capped & tailed mRNA per reaction
    • Streamlined workflow – mRNA capping, DNA removal, mRNA tailing & purification in 2 hrs
    • Flexibility – Enables partial incorporation of 5mCTP, Pseudo-UTP and other modified CTPs and UTPs
    • High quality mRNAs – Ultra high quality components ensure mRNA integrity
    • ARCA-based – Capping in correct orientation ensures high translation efficiency
    • Complete system – Template removal and mRNA purification reagents included
    • More reactions – Twice the number of reactions as compared to competitors’ kits

    HiScribe T7 Quick High Yield RNA Synthesis Kit

    The HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB #E2050) is designed for quick set-up and production of large amounts of RNA in vitro. The reaction can be set up conveniently by combining the NTP buffer mix, T7 RNA Polymerase mix and a suitable DNA template. The kit also allows for capped RNA or dye-labeled RNA synthesis by incorporation of cap analog (ARCA, NEB #S1411) or dye-modified nucleotides. RNA synthesized with the kit can be used for RNA structure and function studies, ribozyme biochemistry, as probes for RNase protection assays and hybridization based blots, anti-sense RNA and RNAi experiments, microarray analysis and microinjection, as well as in vitro translation and RNA vaccines.

    Advantages of the HiScribe T7 Quick High Yield RNA Synthesis Kit

    • Quicker setup
    • Enables synthesis of large amounts of RNA
    • Shelf life is 1 year or longer

    HiScribe T7 High Yield RNA Synthesis Kit

    The T7 High Yield RNA Synthesis Kit (NEB #E2040) is an extremely flexible system for in vitro transcription of RNA using T7 RNA polymerase. The kit allows for synthesis of many kinds of RNA including internally labeled and co-transcriptionally capped transcripts. RNA synthesized from the kit is suitable for many applications including RNA structure and function studies, ribozyme biochemistry, probes for RNase protection assays and hybridization-based blots, anti-sense RNA and RNAi experiments, microarray analysis, microinjection and in vitro translation and RNA vaccines.

    Advantages of the HiScribe T7 High Yield RNA Synthesis Kit

    • Streamlined format
    • Flexibility for high yield production of long and short RNA (up to 180 μg of RNA from 1 μg of control template)
    • Shelf life is 1 year or longer

    HiScribe™ is a trademark of New England Biolabs, Inc.