• My NEB
  • Print
  • PDF
  • Restriction Endonucleases: Molecular Cloning and Beyond


    History of Cloning

    Download image as a PDF

    Learn more about the various types of molecular cloning found in the workflow below:  Traditional Cloning, PCR Cloning, Seamless Cloning, Ligation Independent Cloning (LIC) and Recombinational Cloning.

    Cloning Workflow

    Download image as a PDF

    Synthetic Biology 
    Synthetic Biology is a more recent expansion of the biotechnology field, in which genes and proteins are viewed as parts or devices, with the goal of re-designing and/or assembling these parts in novel ways to create a new and useful functionality. Recent advances in biofuels generation, production of biochemicals, and understanding the minimal genome all benefit from synthetic biological approaches. Often these projects rely on the ordered assembly of multiple DNA sequences to create large, artificial DNA structures. To this end, methods have evolved to simplify this process. One in particular, the Gibson Assembly® method, is credited with "jumpstarting" the field. This method has been used to create many functional DNA structures, from a simple joining of two metabolic genes, all the way up to the creation of an artificial genome.