Restriction Endonucleases
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  • Restriction Endonucleases

    The Leader in the Discovery and Production of Restriction Enzymes

    With over 40 years of offering restriction enzymes to the research community, NEB has earned the reputation of being a leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled performance.

    NEB scientists continue to improve its portfolio of restriction enzymes, as well as explore their utility in new technologies. As a result, NEB scientists continue to publish scientific papers and to be awarded grants in this area. With the industry’s largest research and development group dedicated to restriction enzymes, we are proud to have been there first: the first to commercialize a recombinant enzyme, the first to introduce a nicking enzyme, and the first to supply a true restriction enzyme master mix. In addition, NEB has an ongoing history of innovation by engineering restriction enzymes with altered specificities and improved performance. Through continued research in these areas, we are committed to driving the innovations that allow us to offer maximum convenience and performance.

    For details on NEB’s quality controls for restriction endonucleases, visit our Restriction Enzyme Quality page.

    All of NEB's Restriction enzymes have transitioned to a new buffer system. Visit for further details.



    • Choose a High-Fidelity (HF®) restriction enzyme, which has been engineered for reduced star activity, rapid digestion (5-15 minutes) and 100% activity in CutSmart Buffer. A vial of 6X Purple Loading Dye is included with every HF restriction enzyme.
    • All of our restriction enzymes undergo stringent quality control testing, ensuring the highest levels of purity and lot-to-lot consistency.

    Use Enzyme Finder to select restriction enzyme by name, sequence, overhang or type. 

    1. Type II Restriction Enzymes

      Type II restriction enzymes are most commonly used for molecular biology applications, as they recognize stereotypical sequences and produce a predictable cleavage pattern. Learn more about how Type II REs work.

    2. Type I Restriction Enzymes

      Type I restriction enzymes are a group of endonucleases that recognize a bipartite sequence, but do not produce a predictable cleavage pattern. Learn more about how Type I REs work.

    3. Type III Restriction Enzymes

      Type III restriction enzymes are a group of endonucleases that recognize a non-pallindromic sequence, comprising two inversely oriented sites. Learn more about these poorly understood enzymes.

    4. Cloning With Restriction Enzymes

      Restriction enzymes are an integral part of the cloning workflow, for generating compatible ends on fragments and vectors. This animation discusses three guidelines for determining which restriction enzymes to use in your cloning experiment.

    5. CutSmart™ Restriction Enzyme Buffer

      205 of NEB's restriction enzymes are 100% active in a single buffer. Learn more about CutSmart™ Buffer and why it matters to you.

    6. What Are Restriction Enzymes?

      Watch as Geoff Wilson, Restriction Enzymes Division Head, describes what restriction enzymes are and how they revolutionized molecular biology.

    7. Time-Saver™ Qualified Restriction Enzymes

      How will Time-Saver™ qualified enzymes save you time? Find out from an NEB scientist.

    8. Standard Protocol for Restriction Enzyme Digests

      Let one of NEB's restriction enzyme experts help you improve your technique and avoid common mistakes in digest setup.

    9. What is Restriction Enzyme Star Activity?

      Learn what Star Activity is, why it is detrimental to accurate restriction enzyme digestion, and how NEB's HF™ enzymes are engineered to avoid it.

    10. 6 Reasons to Try RE-Mix Restriction Enzyme Master Mixes

    11. Reduce Star Activity with High-Fidelity Restriction Enzymes

      NEB has engineered HF™ enzymes to eliminate star activity. Learn how, and what this means for your digests.

    12. The Interaction of Restriction Enzymes and DNA

      Watch as Geoff Wilson, Restriction Enzyme Division Head, describes the interaction of restriction enzymes and substrate DNA using computer models generated from x-ray crystallography data.

    13. Why is My Restriction Enzyme Not Cutting DNA

      Not getting the cleavage you expected? Let an NEB scientist help you troubleshoot your reaction.

    14. Restriction Enzyme Digest Problem: Too Many DNA Bands

      Are you finding unexpected bands in your digestion reaction? Here are some tips to help you determine the cause.

    15. Discovering New Restriction Enzymes

      Watch as Rick Morgan, Research Scientist in the Restriction Enzyme Division, describes his passion for discovering and characterizing restriction enzymes from nature.

    16. Setting-up Restriction Enzyme Digests with RE-Mix® Master Mixes

      RE-Mix® Restriction Enzyme Master Mixes offer simplified reaction setup. Learn more about digesting DNA with RE-Mix.

    Restriction Endonucleases includes these subcategories:

    Restriction Endonucleases

    New Restriction Enzyme Trading Cards