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    Restriction EndonucleasesReturn to High-Fidelity (HF®) Restriction Endonucleases

    All High Fidelity (HF™) Restriction Enzymes are Time-Saver qualified and certified to be nuclease free, giving them the power to digest in 5-15 minutes and the flexibility to withstand overnight digestions. Choose the reaction time that works for you. For your convenience, NEB offers over 180 Time-Saver qualified enzymes.

    EcoRI-HF™ (NEB #R3101) shows no star activity when used in 5 minutes or overnight. 50 μl reactions were set up using 1 μg of Lambda DNA, 1 μl of enzyme and recommended reaction buffer. Digests were incubated at 37°C. Marker M is the 1 kb DNA Ladder (NEB# N3232)

    HF™ is a trademark of New England Biolabs, Inc.
    FastDigest® is a registered trademark of Fermentas.

    Learn about High-Fidelity (HF®) Restriction Endonucleases