Restriction Endonucleases

HF®, Nicking, Time-Saver & Other

The Leader in the Discovery and Production of Restriction Enzymes

After more than 40 years of offering restriction enzymes to the research community, NEB has earned the reputation of being a leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled performance.

NEB scientists continue to improve its portfolio of restriction enzymes, as well as explore their utility in new technologies. As a result, NEB scientists continue to publish scientific papers and to be awarded grants in this area. We are proud to have been there first: the first to commercialize a recombinant enzyme, the first to introduce a nicking enzyme, and the first to supply a true restriction enzyme master mix. In addition, NEB has an ongoing history of innovation by engineering restriction enzymes with altered specificities and improved performance. Through continued research in these areas, we are committed to driving the innovations that allow us to offer maximum convenience and performance.

HF®, Nicking, Time-Saver & Other

High-Fidelity (HF®) Restriction Endonucleases

Close

OrderAgeI-HF®

Close

OrderApoI-HF®

Close

OrderBamHI-HF®

Close

OrderBbsI-HF®

Close

OrderBclI-HF

Close

OrderBmtI-HF®

Close

OrderBsaI-HF®

Close

OrderBsiWI-HF®

Close

OrderBsrGI-HF®

Close

OrderBstEII-HF®

Close

OrderBstZ17I-HF®

Close

OrderDraIII-HF®

Close

OrderEagI-HF®

Close

OrderEcoRI-HF®

Close

OrderEcoRV-HF®

Close

OrderHindIII-HF®

Close

OrderKpnI-HF®

Close

OrderMfeI-HF®

Close

OrderMluI-HF®

Close

OrderNcoI-HF®

Close

OrderNheI-HF®

Close

OrderNotI-HF®

Close

OrderNruI-HF®

Close

OrderNsiI-HF®

Close

OrderPstI-HF®

Close

OrderPvuI-HF®

Close

OrderPvuII-HF®

Close

OrderSacI-HF®

Close

OrderSalI-HF®

Close

OrderSbfI-HF®

Close

OrderScaI-HF®

Close

OrderSpeI-HF®

Close

OrderSphI-HF®

Close

OrderSspI-HF®

Close

OrderStyI-HF®

Homing Endonucleases

Close

OrderI-CeuI

Close

OrderI-SceI

Close

OrderPI-PspI

Close

OrderPI-SceI

Nicking Endonucleases

Close

OrderNb.BbvCI

Close

OrderNb.BsmI

Close

OrderNb.BsrDI

Close

OrderNb.BssSI

Close

OrderNb.BtsI

Close

OrderNt.AlwI

Close

OrderNt.BbvCI

Close

OrderNt.BsmAI

Close

OrderNt.BspQI

Close

OrderNt.BstNBI

Restriction Enzymes for Epigenetic Analysis (EpiMark® Validated)

Close

OrderAbaSI

Close

OrderDpnI

Close

OrderDpnII

Close

OrderFspEI

Close

OrderHpaII

Close

OrderLpnPI

Close

OrderMcrBC

Close

OrderMspI

Close

OrderMspJI

Time-Saver™ Qualified Restriction Enzymes

Close

OrderAatII

Close

OrderAcc65I

Close

OrderAccI

Close

OrderAciI

Close

OrderAclI

Close

OrderAcuI

Close

OrderAflII

Close

OrderAgeI-HF®

Close

OrderAhdI

Close

OrderAluI

Close

OrderAlwNI

Close

OrderApaI

Close

OrderApaLI

Close

OrderApeKI

Close

OrderApoI

Close

OrderApoI-HF®

Close

OrderAscI

Close

OrderAseI

Close

OrderAvaI

Close

OrderAvaII

Close

OrderAvrII

Close

OrderBaeGI

Close

OrderBaeI

Close

OrderBamHI

Close

OrderBamHI-HF®

Close

OrderBbsI

Close

OrderBbsI-HF®

Close

OrderBbvI

Close

OrderBciVI

Close

OrderBclI

Close

OrderBclI-HF

Close

OrderBcoDI

Close

OrderBfuAI

Close

OrderBfuCI

Close

OrderBglI

Close

OrderBglII

Close

OrderBlpI

Close

OrderBmgBI

Close

OrderBmtI-HF®

Close

OrderBpmI

Close

OrderBpuEI

Close

OrderBsaAI

Close

OrderBsaHI

Close

OrderBsaI-HF®

Close

OrderBsaWI

Close

OrderBsaXI

Close

OrderBseRI

Close

OrderBsgI

Close

OrderBsiEI

Close

OrderBsiWI

Close

OrderBsiWI-HF®

Close

OrderBslI

Close

OrderBsmAI

Close

OrderBsmBI

Close

OrderBsmI

Close

OrderBsoBI

Close

OrderBsp1286I

Close

OrderBspCNI

Close

OrderBspEI

Close

OrderBspHI

Close

OrderBspQI

Close

OrderBsrBI

Close

OrderBsrDI

Close

OrderBsrGI

Close

OrderBsrI

Close

OrderBssHII

Close

OrderBstBI

Close

OrderBstEII

Close

OrderBstEII-HF®

Close

OrderBstNI

Close

OrderBstUI

Close

OrderBstXI

Close

OrderBstYI

Close

OrderBstZ17I-HF®

Close

OrderBsu36I

Close

OrderBtgI

Close

OrderBtsCI

Close

OrderCac8I

Close

OrderClaI

Close

OrderCspCI

Close

OrderCviAII

Close

OrderCviQI

Close

OrderDdeI

Close

OrderDpnI

Close

OrderDpnII

Close

OrderDraI

Close

OrderDraIII-HF®

Close

OrderDrdI

Close

OrderEagI

Close

OrderEagI-HF®

Close

OrderEarI

Close

OrderEco53kI

Close

OrderEcoNI

Close

OrderEcoO109I

Close

OrderEcoP15I

Close

OrderEcoRI

Close

OrderEcoRI-HF®

Close

OrderEcoRV

Close

OrderEcoRV-HF®

Close

OrderFnu4HI

Close

OrderFseI

Close

OrderFspI

Close

OrderHaeIII

Close

OrderHhaI

Close

OrderHincII

Close

OrderHindIII-HF®

Close

OrderHinfI

Close

OrderHinP1I

Close

OrderHpaII

Close

OrderHphI

Close

OrderHpy166II

Close

OrderHpyAV

Close

OrderHpyCH4IV

Close

OrderHpyCH4V

Close

OrderKpnI

Close

OrderKpnI-HF®

Close

OrderMboI

Close

OrderMfeI-HF®

Close

OrderMluCI

Close

OrderMluI

Close

OrderMlyI

Close

OrderMmeI

Close

OrderMnlI

Close

OrderMseI

Close

OrderMslI

Close

OrderMspA1I

Close

OrderMspI

Close

OrderMwoI

Close

OrderNciI

Close

OrderNcoI

Close

OrderNcoI-HF®

Close

OrderNdeI

Close

OrderNgoMIV

Close

OrderNheI

Close

OrderNheI-HF®

Close

OrderNlaIII

Close

OrderNotI

Close

OrderNotI-HF®

Close

OrderNruI

Close

OrderNsiI

Close

OrderNspI

Close

OrderPacI

Close

OrderPaeR7I

Close

OrderPflFI

Close

OrderPflMI

Close

OrderPmeI

Close

OrderPmlI

Close

OrderPpuMI

Close

OrderPshAI

Close

OrderPstI

Close

OrderPstI-HF®

Close

OrderPvuI

Close

OrderPvuI-HF®

Close

OrderPvuII

Close

OrderPvuII-HF®

Close

OrderRsaI

Close

OrderSacI

Close

OrderSacI-HF®

Close

OrderSacII

Close

OrderSalI

Close

OrderSalI-HF®

Close

OrderSapI

Close

OrderSbfI

Close

OrderSbfI-HF®

Close

OrderScaI-HF®

Close

OrderSfiI

Close

OrderSfoI

Close

OrderSmaI

Close

OrderSpeI

Close

OrderSpeI-HF®

Close

OrderSphI-HF®

Close

OrderSspI-HF®

Close

OrderStuI

Close

OrderStyD4I

Close

OrderStyI

Close

OrderStyI-HF®

Close

OrderSwaI

Close

OrderTaqαI

Close

OrderTfiI

Close

OrderTseI

Close

OrderTspMI

Close

OrderTspRI

Close

OrderTth111I

Close

OrderXbaI

Close

OrderXhoI

Close

OrderXmaI

Close

OrderXmnI

High-Fidelity (HF®) Restriction Endonucleases

Close

OrderAgeI-HF®

Close

OrderApoI-HF®

Close

OrderBamHI-HF®

Close

OrderBbsI-HF®

Close

OrderBclI-HF

Close

OrderBmtI-HF®

Close

OrderBsaI-HF®

Close

OrderBsiWI-HF®

Close

OrderBsrGI-HF®

Close

OrderBstEII-HF®

Close

OrderBstZ17I-HF®

Close

OrderDraIII-HF®

Close

OrderEagI-HF®

Close

OrderEcoRI-HF®

Close

OrderEcoRV-HF®

Close

OrderHindIII-HF®

Close

OrderKpnI-HF®

Close

OrderMfeI-HF®

Close

OrderMluI-HF®

Close

OrderNcoI-HF®

Close

OrderNheI-HF®

Close

OrderNotI-HF®

Close

OrderNruI-HF®

Close

OrderNsiI-HF®

Close

OrderPstI-HF®

Close

OrderPvuI-HF®

Close

OrderPvuII-HF®

Close

OrderSacI-HF®

Close

OrderSalI-HF®

Close

OrderSbfI-HF®

Close

OrderScaI-HF®

Close

OrderSpeI-HF®

Close

OrderSphI-HF®

Close

OrderSspI-HF®

Close

OrderStyI-HF®

Homing Endonucleases

Close

OrderI-CeuI

Close

OrderI-SceI

Close

OrderPI-PspI

Close

OrderPI-SceI

Nicking Endonucleases

Close

OrderNb.BbvCI

Close

OrderNb.BsmI

Close

OrderNb.BsrDI

Close

OrderNb.BssSI

Close

OrderNb.BtsI

Close

OrderNt.AlwI

Close

OrderNt.BbvCI

Close

OrderNt.BsmAI

Close

OrderNt.BspQI

Close

OrderNt.BstNBI

Restriction Enzymes for Epigenetic Analysis (EpiMark® Validated)

Close

OrderAbaSI

Close

OrderDpnI

Close

OrderDpnII

Close

OrderFspEI

Close

OrderHpaII

Close

OrderLpnPI

Close

OrderMcrBC

Close

OrderMspI

Close

OrderMspJI

Time-Saver™ Qualified Restriction Enzymes

Close

OrderAatII

Close

OrderAcc65I

Close

OrderAccI

Close

OrderAciI

Close

OrderAclI

Close

OrderAcuI

Close

OrderAflII

Close

OrderAgeI-HF®

Close

OrderAhdI

Close

OrderAluI

Close

OrderAlwNI

Close

OrderApaI

Close

OrderApaLI

Close

OrderApeKI

Close

OrderApoI

Close

OrderApoI-HF®

Close

OrderAscI

Close

OrderAseI

Close

OrderAvaI

Close

OrderAvaII

Close

OrderAvrII

Close

OrderBaeGI

Close

OrderBaeI

Close

OrderBamHI

Close

OrderBamHI-HF®

Close

OrderBbsI

Close

OrderBbsI-HF®

Close

OrderBbvI

Close

OrderBciVI

Close

OrderBclI

Close

OrderBclI-HF

Close

OrderBcoDI

Close

OrderBfuAI

Close

OrderBfuCI

Close

OrderBglI

Close

OrderBglII

Close

OrderBlpI

Close

OrderBmgBI

Close

OrderBmtI-HF®

Close

OrderBpmI

Close

OrderBpuEI

Close

OrderBsaAI

Close

OrderBsaHI

Close

OrderBsaI-HF®

Close

OrderBsaWI

Close

OrderBsaXI

Close

OrderBseRI

Close

OrderBsgI

Close

OrderBsiEI

Close

OrderBsiWI

Close

OrderBsiWI-HF®

Close

OrderBslI

Close

OrderBsmAI

Close

OrderBsmBI

Close

OrderBsmI

Close

OrderBsoBI

Close

OrderBsp1286I

Close

OrderBspCNI

Close

OrderBspEI

Close

OrderBspHI

Close

OrderBspQI

Close

OrderBsrBI

Close

OrderBsrDI

Close

OrderBsrGI

Close

OrderBsrI

Close

OrderBssHII

Close

OrderBstBI

Close

OrderBstEII

Close

OrderBstEII-HF®

Close

OrderBstNI

Close

OrderBstUI

Close

OrderBstXI

Close

OrderBstYI

Close

OrderBstZ17I-HF®

Close

OrderBsu36I

Close

OrderBtgI

Close

OrderBtsCI

Close

OrderCac8I

Close

OrderClaI

Close

OrderCspCI

Close

OrderCviAII

Close

OrderCviQI

Close

OrderDdeI

Close

OrderDpnI

Close

OrderDpnII

Close

OrderDraI

Close

OrderDraIII-HF®

Close

OrderDrdI

Close

OrderEagI

Close

OrderEagI-HF®

Close

OrderEarI

Close

OrderEco53kI

Close

OrderEcoNI

Close

OrderEcoO109I

Close

OrderEcoP15I

Close

OrderEcoRI

Close

OrderEcoRI-HF®

Close

OrderEcoRV

Close

OrderEcoRV-HF®

Close

OrderFnu4HI

Close

OrderFseI

Close

OrderFspI

Close

OrderHaeIII

Close

OrderHhaI

Close

OrderHincII

Close

OrderHindIII-HF®

Close

OrderHinfI

Close

OrderHinP1I

Close

OrderHpaII

Close

OrderHphI

Close

OrderHpy166II

Close

OrderHpyAV

Close

OrderHpyCH4IV

Close

OrderHpyCH4V

Close

OrderKpnI

Close

OrderKpnI-HF®

Close

OrderMboI

Close

OrderMfeI-HF®

Close

OrderMluCI

Close

OrderMluI

Close

OrderMlyI

Close

OrderMmeI

Close

OrderMnlI

Close

OrderMseI

Close

OrderMslI

Close

OrderMspA1I

Close

OrderMspI

Close

OrderMwoI

Close

OrderNciI

Close

OrderNcoI

Close

OrderNcoI-HF®

Close

OrderNdeI

Close

OrderNgoMIV

Close

OrderNheI

Close

OrderNheI-HF®

Close

OrderNlaIII

Close

OrderNotI

Close

OrderNotI-HF®

Close

OrderNruI

Close

OrderNsiI

Close

OrderNspI

Close

OrderPacI

Close

OrderPaeR7I

Close

OrderPflFI

Close

OrderPflMI

Close

OrderPmeI

Close

OrderPmlI

Close

OrderPpuMI

Close

OrderPshAI

Close

OrderPstI

Close

OrderPstI-HF®

Close

OrderPvuI

Close

OrderPvuI-HF®

Close

OrderPvuII

Close

OrderPvuII-HF®

Close

OrderRsaI

Close

OrderSacI

Close

OrderSacI-HF®

Close

OrderSacII

Close

OrderSalI

Close

OrderSalI-HF®

Close

OrderSapI

Close

OrderSbfI

Close

OrderSbfI-HF®

Close

OrderScaI-HF®

Close

OrderSfiI

Close

OrderSfoI

Close

OrderSmaI

Close

OrderSpeI

Close

OrderSpeI-HF®

Close

OrderSphI-HF®

Close

OrderSspI-HF®

Close

OrderStuI

Close

OrderStyD4I

Close

OrderStyI

Close

OrderStyI-HF®

Close

OrderSwaI

Close

OrderTaqαI

Close

OrderTfiI

Close

OrderTseI

Close

OrderTspMI

Close

OrderTspRI

Close

OrderTth111I

Close

OrderXbaI

Close

OrderXhoI

Close

OrderXmaI

Close

OrderXmnI

Can't find what you need?