Restriction Endonucleases
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  • Restriction Endonucleases

    Cut Smarter with Restriction Enzymes from NEB

    After nearly 40 years of offering restriction enzymes to the research community, NEB has earned the reputation of being a leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled performance.

    Convenience


    Performance

    • Choose a High-Fidelity (HF™) restriction enzyme, which has been engineered for reduced star activity, rapid digestion (5-15 minutes) and 100% activity in CutSmart Buffer.
    • All of our restriction enzymes undergo stringent quality control testing, ensuring the highest levels of purity and lot-to-lot consistency.


    Use Enzyme Finder to select restriction enzymes by name, sequence, overhang or type.



    • CutSmart™ Restriction Enzyme Buffer

      Over 200 of NEB's restriction enzymes are 100% active in a single buffer. Learn more about CutSmart™ Buffer and why it matters to you.

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    • What are Restriction Enzymes?


      Watch as Geoff Wilson, Restriction Enzymes Division Head, describes what restriction enzymes are and how they revolutionized molecular biology.

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    • Time-Saver™ Qualified Restriction Enzymes

      How will Time-Saver™ qualified enzymes save you time? Find out from an NEB scientist.

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    • Reduce Star Activity with High-Fidelity Restriction Enzymes

      NEB has engineered HF™ enzymes to eliminate star activity. Learn how, and what this means for your digests.

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    • Standard Protocol for Restriction Enzyme Digests

      Let one of NEB's restriction enzyme experts help you improve your technique and avoid common mistakes in digest setup.

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    • 6 Reasons to Try RE-Mix® Restriction Enzyme Master Mixes

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    • Why is My Restriction Enzyme Not Cutting DNA?

      Not getting the cleavage you expected? Let an NEB scientist help you troubleshoot your reaction.

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    • Restriction Enzyme Digest Problem: Too Many DNA Bands

      Are you finding unexpected bands in your digestion reaction? Here are some tips to help you determine the cause.

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    • Setting-up Restriction Enzyme Digests with RE-Mix® Master Mixes

      RE-Mix® Restriction Enzyme Master Mixes offer simplified reaction setup. Learn more about digesting DNA with RE-Mix.

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    • Restriction Enzyme Digest Protocol: Cutting Close to DNA End

      When cutting close to the end of a DNA molecule, make sure you know how many bases to add to the ends of your PCR primers.

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    • Cloning With Restriction Enzymes

      Restriction enzymes are an integral part of the cloning workflow, for generating compatible ends on fragments and vectors. This animation discusses three guidelines for determining which restriction enzymes to use in your cloning experiment.

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    Restriction Endonucleases

    Restriction Endonucleases: A

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    Restriction Endonucleases: B

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    Restriction Endonucleases: C-G

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    Restriction Endonucleases: H-M

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    Restriction Endonucleases: N-O

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    Restriction Endonucleases: P-R

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    Restriction Endonucleases: S

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    Restriction Endonucleases: T-Z

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    HF®, Nicking, Master Mix, Time-Saver & Other

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    Restriction Endonuclease Buffers & Diluents

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    Restriction Endonucleases: A

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    Restriction Endonucleases: B

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    Restriction Endonucleases: H-M

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    Restriction Endonucleases: N-O

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    Restriction Endonucleases: P-R

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    Restriction Endonucleases: S

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    Restriction Endonucleases: T-Z

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    HF®, Nicking, Master Mix, Time-Saver & Other

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    Homing Endonucleases

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    Restriction Endonuclease Buffers & Diluents

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