Type II restriction enzymes are most commonly used for molecular biology applications, as they recognize stereotypical sequences and produce a predictable cleavage pattern. Learn more about how Type II REs work.
Type I restriction enzymes are a group of endonucleases that recognize a bipartite sequence, but do not produce a predictable cleavage pattern. Learn more about how Type I REs work.
Type III restriction enzymes are a group of endonucleases that recognize a non-pallindromic sequence, comprising two inversely oriented sites. Learn more about these poorly understood enzymes.
205 of NEB's restriction enzymes are 100% active in a single buffer. Learn more about CutSmart™ Buffer and why it matters to you.
Watch as Geoff Wilson, Restriction Enzymes Division Head, describes what restriction enzymes are and how they revolutionized molecular biology.
How will Time-Saver™ qualified enzymes save you time? Find out from an NEB scientist.
NEB has engineered HF™ enzymes to eliminate star activity. Learn how, and what this means for your digests.
Let one of NEB's restriction enzyme experts help you improve your technique and avoid common mistakes in digest setup.
Not getting the cleavage you expected? Let an NEB scientist help you troubleshoot your reaction.
Are you finding unexpected bands in your digestion reaction? Here are some tips to help you determine the cause.
RE-Mix® Restriction Enzyme Master Mixes offer simplified reaction setup. Learn more about digesting DNA with RE-Mix.
When cutting close to the end of a DNA molecule, make sure you know how many bases to add to the ends of your PCR primers.
Restriction enzymes are an integral part of the cloning workflow, for generating compatible ends on fragments and vectors. This animation discusses three guidelines for determining which restriction enzymes to use in your cloning experiment.