Multiplex PCR can simultaneously detect two or more products in a single reaction. Multiplex PCR can also be used for semi-quantitative gene expression analysis using cDNA templates. The NEB Multiplex PCR 5X Master Mix (NEB #M0284) is an easy-to-use solution featuring high quality recombinant Taq DNA Polymerase, buffer and dNTPs. The mix is optimized for high yield and robust performance. Its performance is illustrated below in a 15-plex PCR using human genomic DNA. The 5X formulation allows the user maximal flexibility for input of primers and template DNAs.