Polymerases and Amplification Technologies
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  • PCR, Polymerases & Amplification Technologies

    NEB supplies DNA polymerases with a variety of properties, such as temperature preference, proofreading exonuclease activity, processivity and strand displacement. In order to facilitate matching the ideal DNA polymerase to a particular research application, the PCR Selection Tool and accompanying text describes the properties associated with specific enzymes.

    1. Overview of PCR

      This overview will walk you through how the Polymerase Chain Reaction (PCR) works.

    PCR, Polymerases & Amplification Technologies includes these subcategories:

    Q5® High-Fidelity DNA Polymerases
    OneTaq® DNA Polymerases
    Taq DNA Polymerases
    Other PCR Polymerases
    Phusion® High-Fidelity DNA Polymerases
    Master Mixes
    DNA Manipulation
    Isothermal Amplification & Strand Displacement
    Nucleotide Solutions
    cDNA Synthesis & Reverse Transcriptases
    Library Preparation

    DNA Polymerase Selection Chart

    NEB offers a guidelines for choosing the correct DNA polymerase for your application by providing a list of specific properites.
    Several factors govern which polymerase should be used in a given application, including:
    Template/product specificity: Is RNA or DNA involved? Is the 3´ terminus at a gap, nick or at the end of the template?
    Removal of existing nucleotides: Will the nucleotide(s) be removed from the existing polynucleotide chain as part of the protocol? If so, will they be removed from the 5´ or the 3´ end?
    Thermal stability: Does the polymerase need to survive incubation at high temperature or is heat inactivation desirable?
    Fidelity: Will subsequent sequence analysis or expression depend on the fidelity of the synthesized products?

    PCR Selection Tool

    Choose from one of the largest selections of polymerases for PCR applications from the leader in enzyme technology and bring unparalleled confidence to your experiments.