Polymerases and Amplification Technologies
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  • Master Mixes

    Featured Products

    For added convenience, many PCR polymerases from NEB are available in a master mix format. Each master mix contains the polymerase, dNTPs, buffer and other components optimized for each particular reaction. Options include OneTaq®, Taq, Quick-Load® Taq (NEB #M0271), Multiplex PCR (NEB #M0284), LongAmp® Taq (NEB #M0287) and LongAmp Hot Start Taq (NEB #M0533) in various concentrations and optimized buffer compositions. Phusion® and Q5™ master mixes bring higher fidelity to PCR reactions, while NEBNext® High-Fidelity 2X PCR Master Mix (NEB #M0541) is specifically optimized for amplification of next-generation sequencing libraries.

    OneTaq®, Quick-Load®, LongAmp® and NEBNext® are registered trademarks of New England Biolabs, Inc.
    Phusion® was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific.
    Q5™ is a trademark of New England Biolabs, Inc.

    DNA Polymerase Selection Chart

    NEB offers a guidelines for choosing the correct DNA polymerase for your application by providing a list of specific properites.

    Several factors govern which polymerase should be used in a given application, including:


    Template/product specificity:
    Is RNA or DNA involved? Is the 3´ terminus at a gap, nick or at the end of the template? 

    Removal of existing nucleotides: Will the nucleotide(s) be removed from the existing polynucleotide chain as part of the protocol? If so, will they be removed from the 5´ or the 3´ end? 

    Thermal stability: Does the polymerase need to survive incubation at high temperature or is heat inactivation desirable? 

    Fidelity: Will subsequent sequence analysis or expression depend on the fidelity of the synthesized products?

    PCR Selection Tool

    Choose from one of the largest selections of polymerases for PCR applications from the leader in enzyme technology and bring unparalleled confidence to your experiments.