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  • Library Preparation

    NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications, such as next generation sequencing and expression library construction. NEB supplies the polymerases needed for sample amplification as a part of sets, master mixes and modules. These robust reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value.

    NEBNext® is a registered trademark of New England Biolabs, Inc.

    A Breakthrough Method of RNA Sample Preparation

    The common problem of adaptor dimer formation during small RNA library construction can be avoided by using NEBNext® protocols. Learn about this technique, and how it improves both performance and sensitivity in library construction.

    The common problem of adaptor dimer formation during small RNA library construction can be avoided by using NEBNext® protocols. Learn about this technique, and how it improves both performance and sensitivity in library construction.

    Advantages

    • Convenient Formats – All of the required enzymes, buffers and nucleotides are included in our sets and modules; many are available in master mix format. Sets for Small RNA and for Ion Torrent include adaptors and primers. Modules offer the ability to customize sample preparation.
    • Functional Validation – Each reagent set or module is functionally validated by preparation of a genomic DNA library that is then sequenced using the appropriate sequencing platform (Illumina, Roche/454, SOLiD or Ion Torrent) and by preparation of an expression library or single-stranded DNA or by preparation of a library from an accepted RNA reference sample.
    • Stringent Quality Controls – Additional QCs ensure maximum quality and purity.
    • Value Pricing