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  • Bst 2.0 DNA Polymerase

    Polymerases and Amplification TechnologiesReturn to Isothermal Amplification & Strand Displacement

    Bst 2.0 DNA Polymerase (NEB #M0537) is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment (Bst DNA Polymerase, Large Fragment, NEB #M0275) . Bst 2.0 DNA Polymerase posesses 5´→3´ DNA polymerase activity and strong strand-displacement activity but lacks 5´→3´ exonuclease activity. Bst 2.0 DNA Polymerase displays improved amplification speed, yield, salt tolerance and thermostability compared to wild-type Bst DNA Polymerase, Large Fragment.

    Bst 2.0 DNA Polymerase Improves Amplification Speed

    SDA amplification of BRCA1 from HeLa genomic DNA under optimal SDA conditions for each enzyme. Data shows that the reaction reaches threshold faster with Bst 2.0 than with wild-type Bst DNA Polymerase.

    Bst 2.0 DNA Polymerase has a Greater Range of Salt Tolerance

    SDA amplification of BRCA1 from HeLa genomic DNA under optimal SDA temperature conditions for each enzyme, with varying salt concentrations. Data shows that Bst 2.0 performance is minimally affected by salt concentration.