The WarmStart® feature of Bst 2.0 DNA Polymerase (NEB #M0538) is unique among isothermal polymerases. Like “Hot Start” PCR polymerases, this feature prevents activity at temperatures below the optimal reaction temperature. This enables room temperature reaction set up and minimizes undesirable reaction products, thereby increasing reaction efficiencies.
In contrast to chemical modifications or antibodies commonly used with hot start PCR polymerases, NEB’s Bst 2.0 WarmStart DNA Polymerase utilizes aptamer technology. Aptamers are extensively modified, unique oligonucleotides which bind to the polymerase through non-covalent interactions, inhibiting activity at non-permissive temperatures (< 50°C). Additionally, no separate activation step is required for Bst 2.0 WarmStart DNA Polymerase.
While standard formulations of Bst DNA polymerase and homologs add non-templated nucleotides to substrate during room temperature reaction setup, this undesirable activity is eliminated in this WarmStart version.
WarmStart® is a trademark of New England Biolabs, Inc.