NEB offers several reagents for cDNA synthesis upstream of applications such as qPCR and qRT-PCR. For your convenience, reagents are available as kits or standalone products to suit your needs.
T7 (NEB #M0251) and SP6 (NEB #M0207) RNA polymerases are DNA-dependent RNA polymerases that produce DNA-templated RNA transcripts. T7 and SP6 exhibit high specificity for their respective promoters. Both T7 and SP6 can be used for the in vitro synthesis of RNA for a wide variety of applications, including transfection, translation, structural studies and radioactive and non-isotopic probe generation. E. coli Poly(A) Polymerase (NEB #M0276) and Poly(U) Polymerase (NEB #M0337) generate untemplated homoribopolymetric tails on RNA 3’-ends. Both E. coli Poly(A) Polymerase and Poly(U) Polymerase can be used for RNA tailing for reverse transcription or labeling.