Release Date: 10/15/2015
Are you challenged with trying to obtain higher library yields using ever-decreasing input amounts? Each component in the NEBNext Ultra II DNA Library Prep Kit from NEB® has been reformulated, resulting in a several-fold increase in library yield with as little as 500 picograms of input DNA. These advances deliver unprecedented performance, while enabling lower inputs and fewer PCR cycles.
Learn more about how NEBNext Ultra II improves library quality and yield by downloading our technical note.
Release Date: 5/19/2015
Accurate quantitation of next-generation sequencing libraries is essential for maximizing data output and quality from each sequencing run. The NEBNext Library Quant Kit delivers significant improvements to qPCR-based library quantitation for next-gen sequencing.
Plan your qPCR library quant reactions with our convenient online calculations tool, NEBioCalculator.
Release Date: 3/16/2015
Golden Gate Assembly relies on the activity of Type IIS restriction enzymes, which are unique because they cleave outside of their recognition sequence. This convenient enzyme mix enables even faster DNA assembly with less hands-on time.
For more information about the many uses of restriction enzymes in molecular cloning, check out our recent article.
Release Date: 3/11/2015
T3 RNA Polymerase is a DNA-dependant RNA polymerase that is highly specific for T3 phage promoters. This new RNA polymerase has many applications in research and biotechnology.
For help choosing which RNA polymerase to choose, consult our RNA polymerase selection chart.
Release Date: 1/21/2015
The NEBNext FFPE DNA Repair Mix is a cocktail of enzymes formulated to repair DNA, and specifically formulated and validated for repair of FFPE DNA samples.
To learn more about the other DNA repair enzymes offered by NEB, refer to this chart.
Release Date: 1/23/2015
The NEBNext Q5 Hot Start HiFi PCR Master Mix is specifically optimized for robust, high-fidelity amplification of next-generation sequencing (NGS) libraries, regardless of GC content. The polymerase component of the master mix, Q5 High-Fidelity DNA Polymerase, has an ultra-low error rate (>100 fold lower than that of Taq DNA Polymerase).
For tips on optimizing NGS library preparation techniques and yields, view this quick video.