Markers and Ladders
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  • Markers & Ladders

    DNA/RNA Molecular Weight Markers

    New England Biolabs sells a wide range of double-stranded DNA molecular weight markers for conventional electrophoresis. Conventional electrophoresis markers include: HindIII (NEB #N3012) and BstEII (NEB #N3014) digests of Lambda DNA, BstNI (NEB #N3031) and MspI (NEB #N3032) digests of pBR322 DNA, and a HaeIII (NEB #N3026) digest of φX174 DNA. These markers have a size range of approximately 10 base pairs to 23,000 base pairs. Our Lambda DNA–Mono Cut Mix (NEB #N3019) is designed to be used as an RFLP marker on Southern blots because it provides an uncomplicated gel pattern. 

    We also supply a series of DNA ladders ranging from 25 bp to 48.5 kb. Our 100 bp DNA Ladder, 1 kb DNA Ladder and 2-Log DNA Ladder are also available in four formats: Conventional (NEB #N3231, NEB #N3232, NEB #N3200), Quick-Load® (NEB #N0467, NEB #N0468, NEB #N0469) with bromophenol blue tracking dye, Quick Load Purple (NEB #N0550, NEB #N0551, NEB #N0552) using a non-fluorescing, purple dye, and TriDye™ (NEB #N3271, NEB #N3272, NEB #N3270) containing three dyes to track gel migration. NEB's DNA ladders feature exceptional quality and value. With our new Quick-Load Purple DNA Ladders (NEB #N0550, NEB #N0551, NEB #N0552), you'll appreciate the crisp bands and improved visibility, since there's no UV shadow.

    RNA Markers and Ladders

    Our RNA ladders and markers have a size range of up to 9,000 bases. These include the Low Range ssRNA Ladder (NEB #N0364), ssRNA Ladder (NEB #N0362), dsRNA Ladder (NEB #N0363), and microRNA Marker (NEB #N2102). 

    Protein Standards 

    NEB offers a selection of highly pure protein markers and ladders, with a size range of 2.3 to 250 kDa, which is ideal for accurate molecular weight determination for a wide range of expressed proteins. Formats include unstained, and pre-stained (blue or color).

    TriDye™ is a trademark of New England Biolabs.
    Quick-Load® is a registered trademark of New England Biolabs.

    Featured Products

    Markers & Ladders includes these subcategories:

    DNA Markers & Ladders
    Protein Markers & Ladders
    RNA Markers & Ladders

    Protocols

    Advantages of NEB DNA Ladders

    Choose DNA Ladders from NEB for Quality, consistency and value
     
    • Stable at room temperature
    • Sharp uniform bands 
    • Easy-to-identify reference bands 
    • Can be used for sample quantitation 
    • No extra backbone DNA present 
    • Quick-Load® ladders contain bromophenol blue for tracking 
    • TriDye™ ladders contain three dyes to monitor migration

    Advantages of NEB Protein Markers & Ladders

    • Suitable for analysis of a wide range of expressed proteins 
    • Uniform band intensities and convenient band spacing
    • Easy-to-identify reference bands

    DNA Markers & Ladders

    RNA Markers & Ladders

    RNA Markers Gray Thumb

    Protein Markers & Ladders

    Comparison of NEB to Other Commercially Available DNA Ladders

    Comparison of the NEB 1kb (left) and 100bp (right) DNA Ladders to other commercially available DNA Ladders. Note: The 500 bp DNA Ladder from Roche is used in the 1 kb DNA Ladder comparison.

    DNA Markers & Ladders Selection Chart

    NEB offers a variety of DNA ladders with sizes ranging from 25 bp to 40 kb for use in agarose gel electrophoresis. NEB also offers double-stranded DNA molecular weight markers for conventional with sizes ranging from 10 bp to 23,000 base pairs for use in agarose gel electrophoresis.