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  • RNA Reagent Sets

    NEB offers a mRNA Library Preparation Reagent Sets for the Illumina® (NEB #E6100) platform.

    The NEBNext® mRNA Library Prep Reagent Set contains individual enzymes and buffers (not master mixes) that are ideally suited for library preparation for next generation sequencing. Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.

    Illumina® is a registered trademark of Illumina, Inc.
    NEBNext® is a registered trademark of New ENgland Biolabs, Inc.
    1. 12 Quick Tips for NGS Library Preparation

      Using beads to clean up your DNA prior to NGS library prep can be quick and easy, if you follow these few, simple tips.

    2. Which NEBNext Product Format Should I Choose?

      Not sure how sets and kits differ from modules? Ping explains the difference, and helps you to decide which one is right for you.

    3. Tips for Optimizing RNA Inputs in NGS Library Construction

      Watch as Deyra explains how to optimize your RNA inputs for successful NGS library preparation.

    4. Tips for Bead-based Clean ups and Size Selection

      Using beads to clean up your DNA prior to NGS library prep can be quick and easy, if you follow these few, simple tips.

    Advantages of NEBNext Reagents for RNA Library Preparation

    • Convenient Formats – Enzymes, buffers and nucleotides are included, at appropriate concentrations and in appropriate volumes, and are available in set or master mix format. Large and small sizes are available. 
    • Functional Validation – Each set is validated together by preparation of a library from an accepted RNA reference sample, followed by sequencing on the appropriate sequencing platform (Illumina, 454 or SOLiD). 
    • Stringent Quality Controls
    • Value Pricing
    • Novel Protocol for small RNA – Higher yield and substantially reduced adaptor dimer formation suitable for methylated and unmethylated small RNAs

    Applications of NEBNext Reagents for RNA Library Preparation

    • mRNA library preparation for next generation sequencing
    • Small RNA library preparation for sequencing with Illumina and SOLiD
    • Expression library construction

    mRNA Library Construction Workflow

    mRNA Library Construction Workflow for Illumina

    mRNA Library Construction Workflow for 454

    Small RNA Library Construction Workflow

    Small RNA Library Construction Workflow for Illumina

    Small RNA Library Construction Workflow for SOLiD