Epigenetics

Methyltransferases for Epigenetics

DNA methyltransferases are enzymes that modify DNA by adding a methyl group to either cytosines or adenines depending on the specificity of the enzyme. DNA methyltransferases can be used experimentally to methylate DNA at specific sites for gene expression studies. Our selection includes CpG methyltransferase (NEB #M0226), which is especially useful for studying CpG methylation effects. These enzymes are also useful for producing positive controls for methylation-specific PCR or bisulfite sequencing.

Several protein methyltransferases (G9A (NEB #M0235), SET7 (NEB #M0233), PRMT1 (NEB #M0234)) are also available for the methylation of specific lysine and arginine residues in Histone H3 and H4 in gene regulation studies.

Applications

  • Positive controls for methylation specific PCR or bisulfite sequencing
  • CpG-methylated gene expression studies
  • Nucleosome footprinting

Types of Histone Modifications

Amino Acid Modification
Lysine Methylation, Acetylation,
Ubiquitination, Sumoylation,
ADP-Ribosylation
Arginine Methylation
Serine Phosphorylation
Threonine Phosphorylation

Types of DNA Modifications

References

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