The study of chromatin associated proteins, such as histones, their assembly with and modification by enzymatic regulators is an important area of epigenetic research.
Chromatin is organized into nucleosome core particles that consist of approximately 147 bp of DNA and an octamer complex made up of two molecules of each histone (H2A (NEB #M2502), H2B (NEB #M2505), H3 (NEB #M2503, NEB #M2506, NEB #M2507) and H4 (NEB #M2504)). The linker histone, H1 (NEB #M2501), can further condense chromatin by binding to linker DNA between the nucleosome core particles. For histone modification studies, New England Biolabs offers a selection of unmodified, recombinant human histones and the EpiMark® Nucleosome Assembly Kit (NEB #E5350) which contains preformed Histone H2A/H2B Dimer (NEB #M2508) and Histone H3.1/H4 Tetramer (NEB #M2509).
NEB's highly purified, recombinant histones are excellent substrates for the characterization of histone modification enzymes. Mass spectrometry analysis demonstrates that they are free of post-translational modifications. In addition, assembly of recombinant nucleosomes has been greatly simplified by the introduction of the EpiMark® Nucleosome Assembly Kit (NEB #E5350)
EpiMark® is a registered trademark of New England Biolabs, Inc.