Epigenetics
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  • Epigenetics

    New England Biolabs has called upon its 35 years of expertise in enzymology to develop a suite of validated reagents for epigenetics research. This line of easy-to-use EpiMark® kits simplifies DNA methylation and hydroxymethylation detection and analysis, as well as ChIP, histone and nucleosome analysis. Independently applicable, individual epigenetics reagents also complement the EpiMark® kits. NEB's methylation- and hydroxymethylation- sensitive or dependent enzymes, DNA methyltransferases and DNA controls are all useful for mapping DNA modifications and methylating DNA at specific sites for gene expression studies. Our protein methyltransferases and recombinant histones perform efficiently in protein modification and characterization studies. Our range of modified and unmodified genomic DNAs can be used as controls for detection of DNA methylation. Our series of human DNA (cytosine-5) methyltransferase (DNMT) antibodies are ideally suited for Western blots and immunoprecipitation.

    Our complete Epigenetics suite is expertly designed for optimized research and discovery.

    EpiMark® is a registered trademark of New England Biolabs, Inc.
    • What Is Epigenetics?

      If all cells are created from the same genetic material, why are there so many different cell types? Listen to Sriharsa Pradhan, Senior Scientist, RNA Biology at NEB, as he describes how DNA is methylated and how this affects the path of reading the DNA code the same way an obstruction would derail a train off its tracks.

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    Learn More

    Epigenetics includes these subcategories:

    Antibodies
    Control DNA
    Epigenetic Analysis (Epimark® Validated)
    Histones
    Methylation Dependent Restriction Enzymes
    Methylation-Sensitive Restriction Enzymes
    Methyltransferases

    Types of Histone Modifications

    Amino Acid Modification
    Lysine Methylation, Acetylation,
    Ubiquitination, Sumoylation,
    ADP-Ribosylation
    Arginine Methylation
    Serine Phosphorylation
    Threonine Phosphorylation

    Types of DNA Modifications

    References

    1. Kim, J.K., Samaranayake, M. and Pradhan S. (2009) Cell. Mol. Life Sci. 66, 596-612. PMID: 18985277
    2. Vanyushin, B.F. (2006) Curr. Top. Microbiol. Immunol. 301, 67-122. PMID: 16570846
    3. Mosher, R.A., Melnyk, C.W. (2010) Trends Plant Sci. 15, 204-210. PMID: 20129810
    4. Lyko, F., Beisel, C., Marhold, J., Paro, R. (2006) Curr. Top. Microbiol. Immunol. 310, 23-44. PMID: 16909905
    5. Selker, E.U., Freitag, M., Kothe, G.O., et al. (2002) Proc. Natl. Acad. Sci. U S A. 99, Suppl 4, 16485-16490. PMID: 12189210
    6. Kriaucionis, S. and Heintz, N. (2009) Science 324, 929-930. PMID: 19372393
    7. Tahiliani, M., Koh, K. P., Shen, Y., et al. (2009) Science 324, 930-935. PMID: 19372391
    8. Ehrlich, M., Wilson, G.G., Kuo, K.C., And Gehrke, C.W. (1987) J. Bacteriol. 169, 939-943. PMID: 3029036
    9. Svadbina, I.V., Zelinskaya, N.V., Kovalevskaya, N.P., Zheleznaya, L.A. and Matvienko, N.I. (2004) Biochem. (Moscow) 69, 299-305. PMID: 15061697
    10. Ratel, D., Ravanat, J-L., Berger, F. and Wion D. (2006) Bioessays 28, 309-315. PMID: 16479578
    11. Marinus, M.G. and Casadesus, J. (2009) FEMS Microbiol. Rev. 33, 488-503. PMID: 19175412

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