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  • Restriction Endonucleases

    Synthetic Biology

    The evolution of our understanding of life sciences has opened the doors to recreate natural systems, and even design biological functions and systems that do not exist in nature. NEB supports synthetic biology research by providing the BioBrick® Assembly Kit (NEB #E0546) and the Gibson Assembly™ Master Mix (NEB #E2611). The use of both products is widespread among the synthetic biology community because of their convenience, flexibility and suitability for a wide range of sequence sizes.

    BioBrick® is a registered trademark of the BioBricks Foundation.
    Gibson Assembly™ is a manufactured by New England Biolabs, Inc under licence from synthetic Genomics, Inc. 

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    Advantages

    • Increased number of successful assembly products, particularly for longer or greater number of fragments 
    • Flexible sequence design with no need to engineer cloning sites (scar-less cloning) 
    • Does not require clean up of PCR products prior to assembly 
    • Complex assembly achieved in 1 hour 
    • DNA can be used immediately for transformation, or as template for PCR or RCA 
    • Easily adapted for multiple DNA manipulations, including site-directed mutagenesis, insertions and deletions