DNA Modifying Enzymes

Phosphatases & Sulfurylases

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  • NEB supplies a variety of phosphatases for use in cloning, such as preparation of templates for 5´end labeling, prevention of recircularization of cloning vectors, enhancing yields of RNA in transcription reactions.

    Phosphatases catalyze the removal of 5´ phosphate groups from DNA and RNA (Shrimp Alkaline Phosphatase (NEB #M0371) and Antarctic Phosphatase (NEB #M0289). Inorganic phosphatases catalyze the hydrolysis of inorganic pyrophosphate to form orthophosphate (Pyrophosphatase, Inorganic (yeast) (NEB #M2403) and Thermostable Inorganic Pyrophosphatase (NEB #M0296)).

    ATP Sulfurylase (NEB #M0394) catalyzes the activation of sulfate by transferring sulfate to the adenine monophosphate moiety of ATP to form adenosine 5´-phosphosulfate (APS) and pyrophosphate.

    NEBCloner is a guide for selecting appropriate products and viewing protocols for steps in the cloning workflow. To help select the right DNA phosphatase, choose "End Modification" on the tool to start. 

    1. The Mechanism of Dephosphorylation

      Dephosphorylation is the process by which phosphate groups are removed from a molecule by a phosphatase. Removal of phosphate groups from a DNA fragment can prevent ligation. Learn more about dephosphorylation and phosphatases.

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