DNA Modifying Enzymes
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  • Kinases

    The T4 Polynucleotide Kinase (NEB #M0201) can be used to 5' end label DNA and RNA because it catalyzes the transfer and exchange of phosphate from ATP to the 5´ -hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3´-monophosphates. Polynucleotide Kinase also catalyzes the removal of 3´-phosphoryl groups from 3´-phosphoryl polynucleotides, deoxynucleoside 3´-monophosphates and deoxynucleoside 3´-diphosphates (1). 

    The T4 Polynucleotide Kinase (3' phosphatase minus) (NEB #M0236) is a modified version that exhibits full kinase activity with no 3´ phosphatase activity (2,3).

    References

    1. Richardson, C.C. (1981) P.D. Boyer (Eds.), The Enzymes, 14, pp. 229-314. San Diego: Academic press.
    2. Wang, L.K. and Shuman, S. (2001) J. Biol. Chem., 276, 26868-26874. PMID: 11335730
    3. Wang, L.K. and Shuman, S. (2002) Nucl. Acids Res., 30, 1073-1080. PMID: 11842120

    Common Applications of Exonucleases and Endonucleases

    NEB provides a list of common applications for our exonucleases and endonucleases.

    Properties of Exonucleases and Endonucleases

    NEB supplies many nucleases; several characteristics should be considered when choosing the one best suited to your particular research needs.