Blunt/TA Ligase Master Mix
The Blunt/TA Ligase Master Mix (NEB #M0367) is specifically formulated to improve ligation and transformation of both blunt-end and single-base overhang substrates. This master mix format simplifies reaction setup, ensuring robust, rapid ligation with an optimized ratio of enzyme and buffer components. Reactions can be used to directly transform many strains of chemically competent E. coli without dilution. In this application note, we demonstrate that this mix with proprietary enhancers delivers superior performance when working with blunt or single base-pair fragments.
Instant Sticky-end Ligase Master Mix
Complete with T4 DNA Ligase (NEB #M0202), proprietary ligation enhancer and optimized reaction buffer, the Instant Sticky-end Ligase Master Mix (NEB #M0370) enables instant, incubation- free ligation. Specifically formulated to rapidly ligate sticky-end (2–4 bp) substrates and improve transformation, this mix simplifies reaction set-up and reduces the time needed for routine ligations. Reactions can be used to directly transform many strains of chemically competent E. coli without dilution.
In this application note, we demonstrate the efficient adaptor ligation for dsDNA libraries.