DNA Modifying Enzymes
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  • Cloning & Mutagenesis

    NEB offers a selection of common cloning plasmids and DNAs for use as substrates. We provide several multipurpose cloning plasmids with different promoters and selection genes for your cloning needs.

    Learn how simple it really is to use the Gibson Assembly™ Cloning Kit

    • Introduction to Gibson Assembly®

      Watch an interactive tutorial that details the process by which Gibson Assembly® joins DNA fragments in a single tube, isothermal reaction.

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    • Primer Design and Fragment Assembly Using Gibson Assembly®

      Watch an interactive tutorial on primer design to see how simple it really is to clone with the Gibson Assembly® Cloning Kit.

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    Common Applications of Exonucleases and Endonucleases

    NEB provides a list of common applications for our exonucleases and endonucleases.

    Properties of Exonucleases and Endonucleases

    NEB supplies many nucleases; several characteristics should be considered when choosing the one best suited to your particular research needs.

    Cloning Plasmids Selection Chart

    Benefits of the Gibson Assembly Cloning Kit

    • Assembly and transformation in a little over an hour
    • Flexible sequence design (scar-less cloning)
    • No PCR clean-up step required
    • High transformation efficiencies for inserts up to 20 kb
    • Easily adapted for multiple DNA manipulations, including site-directed mutagenesis
    • Includes competent cells

    Overview of the Gibson Assembly Cloning Method

    Gibson Assembly Cloning Kit Provides Robust Transformation Efficiencies

    Assembly reactions containing 25 ng of linear pUC19 vector and 0.04 pmol of each fragment were performed following individual suppliers’ recommended protocols and using the competent cells provided with the kit. The total number of recombinant colonies was calculated per 25 ng of linear pUC19 vector added to the assembly reaction.