DNA Modifying Enzymes
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  • DNA Modifying Enzymes & Cloning Technologies

    NEB offers a comprehensive list of tools and reagents for DNA and RNA manipulation and cloning. These include ligases, nucleases, methyltransferases, DNA repair proteins, recombinases and more.

    DNA Modifying Enzymes & Cloning Technologies

    DNA Ligases

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    OrderThermostable 5´ AppDNA/RNA Ligase

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    OrderE. coli DNA Ligase

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    OrderTaq DNA Ligase

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    OrderTaq DNA Ligase Reaction Buffer

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    Order9°N™ DNA Ligase

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    OrderBlunt/TA Ligase Master Mix

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    OrderElectroLigase®

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    OrderInstant Sticky-end Ligase Master Mix

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    OrderNEBNext® Quick Ligation Module

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    OrderQuick Blunting™ and Quick Ligation™ Kits

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    OrderQuick Ligation™ Kit

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    OrderSplintR® Ligase

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    OrderT3 DNA Ligase

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    OrderT4 DNA Ligase

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    OrderT4 DNA Ligase Reaction Buffer

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    OrderT7 DNA Ligase

    DNA Repair Proteins

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    OrderAfu Uracil-DNA Glycosylase (UDG)

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    OrderTma Endonuclease III

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    OrderTth Endonuclease IV

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    OrderAntarctic Thermolabile UDG

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    OrderAPE 1

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    OrderDNA Gyrase (E. coli)

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    OrderDNA Gyrase (E. coli) Substrate

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    OrderEndonuclease III (Nth)

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    OrderEndonuclease IV

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    OrderEndonuclease V

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    OrderEndonuclease VIII

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    OrderFpg

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    OrderhAAG

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    OrderhOGG1

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    OrderhSMUG1

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    OrderPreCR® Repair Mix

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    OrderRecA

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    OrderRecAf

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    OrderT4 PDG (T4 Endonuclease V)

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    OrderT7 Endonuclease I

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    OrderTopoisomerase I (E. coli)

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    OrderUDG Reaction Buffer

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    OrderUracil Glycosylase Inhibitor (UGI)

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    OrderUracil-DNA Glycosylase (UDG)

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    OrderUSER™ Enzyme

    Methyltransferases

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    Orderdam Methyltransferase

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    OrderTaqI Methyltransferase

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    OrderAluI Methyltransferase

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    OrderBamHI Methyltransferase

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    OrderCpG Methyltransferase (M.SssI)

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    OrderEcoRI Methyltransferase

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    OrderG9a Methyltransferase

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    OrderGpC Methyltransferase (M.CviPI)

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    OrderHaelll Methyltransferase

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    OrderHhal Methyltransferase

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    OrderHpaII Methyltransferase

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    OrderHuman DNA (cytosine-5) Methyltransferase (Dnmt1)

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    OrderHuman DNA (cytosine-5) Methyltransferase (Dnmt1) Amino-terminal Ab

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    OrderMspI Methyltransferase

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    OrderPRMT1 Methyltransferase

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    OrderSET7 Methyltransferase

    Nucleases

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    OrderAfu Uracil-DNA Glycosylase (UDG)

    Close

    OrderTma Endonuclease III

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    OrderTth Endonuclease IV

    Close

    OrderAntarctic Thermolabile UDG

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    OrderAPE 1

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    OrderCas9 Nuclease, S. pyogenes

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    OrderDNase I (RNase-free)

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    OrderDNase I Reaction Buffer

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    OrderEndonuclease III (Nth)

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    OrderEndonuclease IV

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    OrderEndonuclease V

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    OrderEndonuclease VIII

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    OrderExonuclease I (E. coli)

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    OrderExonuclease I Reaction Buffer

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    OrderExonuclease III (E. coli)

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    OrderExonuclease T

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    OrderExonuclease V (RecBCD)

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    OrderFpg

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    OrderhAAG

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    OrderhOGG1

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    OrderhSMUG1

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    OrderLambda Exonuclease

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    OrderLambda Exonuclease Reaction Buffer

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    OrderMicrococcal Nuclease

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    OrderMung Bean Nuclease

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    OrderNuclease BAL-31

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    OrderPreCR® Repair Mix

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    OrderRecAf

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    OrderRecJf

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    OrderT4 PDG (T4 Endonuclease V)

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    OrderT5 Exonuclease

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    OrderT7 Endonuclease I

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    OrderT7 Exonuclease

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    OrderUracil Glycosylase Inhibitor (UGI)

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    OrderUracil-DNA Glycosylase (UDG)

    Phosphatases & Sulfurylases

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    OrderAlkaline Phosphatase, Calf Intestinal (CIP)

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    OrderAntarctic Phosphatase

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    OrderAntarctic Phosphatase Reaction Buffer

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    OrderApyrase

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    OrderATP Sulfurylase

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    Biotinylated Alkaline Phosphatase

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    OrderPyrophosphatase, Inorganic (E. coli)

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    OrderPyrophosphatase, Inorganic (yeast)

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    OrderShrimp Alkaline Phosphatase (rSAP)

    Close

    OrderThermostable Inorganic Pyrophosphatase

    Recombinases

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    OrderCre Recombinase

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    OrderpLox2+ (linearized)

    ssDNA Binding Proteins

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    OrderET SSB

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    OrderRecA

    Close

    OrderRecAf

    Close

    OrderT4 Gene 32 Protein

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    OrderTth RecA

    Synthetic Biology

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    OrderBioBrick® Assembly Kit

    Close

    OrderGibson Assembly® Cloning Kit

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    OrderGibson Assembly® Master Mix

    Kinases

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    OrderT4 Polynucleotide Kinase

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    OrderT4 Polynucleotide Kinase (3' phosphatase minus)

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    OrderT4 Polynucleotide Kinase Reaction Buffer

    DNA Labeling & Chemiluminescent Detection

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    NEBlot® Kit

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    OrderStreptavidin

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    OrderTerminal Transferase

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    OrderTherminator™ DNA Polymerase

    DNA Library Preparation

    Close

    OrderNEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®

    Close

    OrderNEBNext® ChIP-Seq Library Prep Reagent Set for Illumina®

    Close

    OrderNEBNext® dA-Tailing Module

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    OrderNEBNext® dA-Tailing Reaction Buffer

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    OrderNEBNext® DNA Library Prep Master Mix Set for 454™

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    OrderNEBNext® DNA Library Prep Master Mix Set for Illumina®

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    NEBNext® DNA Library Prep Reagent Set for 454™

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    OrderNEBNext® DNA Library Prep Reagent Set for Illumina®

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    NEBNext® DNA Library Prep Set for SOLiD™

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    OrderNEBNext® dsDNA Fragmentase®

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    OrderNEBNext® End Repair Module

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    OrderNEBNext® End Repair Reaction Buffer

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    OrderNEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent

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    OrderNEBNext® Fast DNA Library Prep Set for Ion Torrent™

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    OrderNEBNext® Microbiome DNA Enrichment Kit

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    OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

    Close

    OrderNEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1)

    Close

    OrderNEBNext® Quick DNA Library Prep Master Mix Set for 454™

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    OrderNEBNext® Quick Ligation Module

    Close

    OrderNEBNext® Quick Ligation Reaction Buffer

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    OrderNEBNext® Singleplex Oligos for Illumina®

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    OrderNEBNext® Ultra™ End Repair/dA-Tailing Module

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    OrderNEBNext® Ultra™ Ligation Module

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    OrderNEBNext® Ultra™ DNA Library Prep Kit for Illumina®

    Cloning & Mutagenesis

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    Orderdam-/dcm- Competent E. coli

    Close

    Order9°N™ DNA Ligase

    Close

    OrderCrimson LongAmp™ Taq DNA Polymerase

    Close

    OrderGibson Assembly® Cloning Kit

    Close

    OrderGibson Assembly® Master Mix

    Close

    OrderKlenow Fragment (3'→5' exo-)

    Close

    OrderLambda DNA

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    OrderLambda DNA (N6-methyladenine-free)

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    OrderLITMUS 28i Vector

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    OrderLongAmp™ Taq DNA Polymerase

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    OrderM13mp18 RF I DNA

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    OrderM13mp18 Single-stranded DNA

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    OrderNEB 10-beta Competent E. coli (High Efficiency)

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    OrderNEB 10-beta Electrocompetent E. coli

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    OrderNEB 5-alpha Competent E. coli (High Efficiency)

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    OrderNEB 5-alpha Competent E. coli (Subcloning Efficiency)

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    OrderNEB 5-alpha Electrocompetent E. coli

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    OrderNEB 5-alpha F'Iq Competent E. coli (High Efficiency)

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    OrderNEB Stable Competent E. coli (High Efficiency)

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    OrderNEB Turbo Competent E. coli (High Efficiency)

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    OrderNEB Turbo Electrocompetent E. coli

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    OrderNEB® Essential Cloning Set

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    OrderNEB® PCR Cloning Kit

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    OrderpBR322 Vector

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    OrderpCLuc Mini-TK 2 Vector

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    OrderpCLuc-Basic 2 Vector

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    OrderpGLuc Mini-TK 2 Vector

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    OrderpGLuc-Basic 2 Vector

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    OrderPhusion® High-Fidelity DNA Polymerase

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    OrderPhusion® High-Fidelity PCR Kit

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    OrderpNEB206A Linearized Vector

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    OrderpSV40-GLuc Control Plasmid

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    OrderpTK-CLuc Vector

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    OrderpTK-GLuc Vector

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    OrderpUC19 Vector

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    OrderQ5® Site-Directed Mutagenesis Kit

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    OrderQ5® Site-Directed Mutagenesis Kit (Without Competent Cells)

    Close

    OrderQuick Blunting™ and Quick Ligation™ Kits

    Close

    OrderQuick Blunting™ Kit

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    OrderQuick Ligation™ Kit

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    OrderT7 DNA Polymerase (unmodified)

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    OrderUSER™ Enzyme

    Close

    OrderΦX174 RF I DNA

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    OrderΦX174 RF II DNA

    Close

    OrderΦX174 Virion DNA

    Other

    Close

    Orderβ-Agarase I

    Close

    Orderβ-Agarase I Reaction Buffer

    Close

    OrderM13KO7 Helper Phage

    DNA Modifying Enzymes & Cloning Technologies Buffers & Diluents

    Close

    Orderβ-Agarase I Reaction Buffer

    Close

    OrderTaq DNA Ligase Reaction Buffer

    Close

    OrderAntarctic Phosphatase Reaction Buffer

    Close

    OrderDNase I Reaction Buffer

    Close

    OrderExonuclease I Reaction Buffer

    Close

    OrderLambda Exonuclease Reaction Buffer

    Close

    OrderT4 DNA Ligase Reaction Buffer

    Close

    OrderT4 Polynucleotide Kinase Reaction Buffer

    Close

    OrderUDG Reaction Buffer

    DNA Ligases

    Close

    OrderThermostable 5´ AppDNA/RNA Ligase

    Close

    OrderE. coli DNA Ligase

    Close

    OrderTaq DNA Ligase

    Close

    OrderTaq DNA Ligase Reaction Buffer

    Close

    Order9°N™ DNA Ligase

    Close

    OrderBlunt/TA Ligase Master Mix

    Close

    OrderElectroLigase®

    Close

    OrderInstant Sticky-end Ligase Master Mix

    Close

    OrderNEBNext® Quick Ligation Module

    Close

    OrderQuick Blunting™ and Quick Ligation™ Kits

    Close

    OrderQuick Ligation™ Kit

    Close

    OrderSplintR® Ligase

    Close

    OrderT3 DNA Ligase

    Close

    OrderT4 DNA Ligase

    Close

    OrderT4 DNA Ligase Reaction Buffer

    Close

    OrderT7 DNA Ligase

    DNA Repair Proteins

    Close

    OrderAfu Uracil-DNA Glycosylase (UDG)

    Close

    OrderTma Endonuclease III

    Close

    OrderTth Endonuclease IV

    Close

    OrderAntarctic Thermolabile UDG

    Close

    OrderAPE 1

    Close

    OrderDNA Gyrase (E. coli)

    Close

    OrderDNA Gyrase (E. coli) Substrate

    Close

    OrderEndonuclease III (Nth)

    Close

    OrderEndonuclease IV

    Close

    OrderEndonuclease V

    Close

    OrderEndonuclease VIII

    Close

    OrderFpg

    Close

    OrderhAAG

    Close

    OrderhOGG1

    Close

    OrderhSMUG1

    Close

    OrderPreCR® Repair Mix

    Close

    OrderRecA

    Close

    OrderRecAf

    Close

    OrderT4 PDG (T4 Endonuclease V)

    Close

    OrderT7 Endonuclease I

    Close

    OrderTopoisomerase I (E. coli)

    Close

    OrderUDG Reaction Buffer

    Close

    OrderUracil Glycosylase Inhibitor (UGI)

    Close

    OrderUracil-DNA Glycosylase (UDG)

    Close

    OrderUSER™ Enzyme

    Methyltransferases

    Close

    Orderdam Methyltransferase

    Close

    OrderTaqI Methyltransferase

    Close

    OrderAluI Methyltransferase

    Close

    OrderBamHI Methyltransferase

    Close

    OrderCpG Methyltransferase (M.SssI)

    Close

    OrderEcoRI Methyltransferase

    Close

    OrderG9a Methyltransferase

    Close

    OrderGpC Methyltransferase (M.CviPI)

    Close

    OrderHaelll Methyltransferase

    Close

    OrderHhal Methyltransferase

    Close

    OrderHpaII Methyltransferase

    Close

    OrderHuman DNA (cytosine-5) Methyltransferase (Dnmt1)

    Close

    OrderHuman DNA (cytosine-5) Methyltransferase (Dnmt1) Amino-terminal Ab

    Close

    OrderMspI Methyltransferase

    Close

    OrderPRMT1 Methyltransferase

    Close

    OrderSET7 Methyltransferase

    Nucleases

    Close

    OrderAfu Uracil-DNA Glycosylase (UDG)

    Close

    OrderTma Endonuclease III

    Close

    OrderTth Endonuclease IV

    Close

    OrderAntarctic Thermolabile UDG

    Close

    OrderAPE 1

    Close

    OrderCas9 Nuclease, S. pyogenes

    Close

    OrderDNase I (RNase-free)

    Close

    OrderDNase I Reaction Buffer

    Close

    OrderEndonuclease III (Nth)

    Close

    OrderEndonuclease IV

    Close

    OrderEndonuclease V

    Close

    OrderEndonuclease VIII

    Close

    OrderExonuclease I (E. coli)

    Close

    OrderExonuclease I Reaction Buffer

    Close

    OrderExonuclease III (E. coli)

    Close

    OrderExonuclease T

    Close

    OrderExonuclease V (RecBCD)

    Close

    OrderFpg

    Close

    OrderhAAG

    Close

    OrderhOGG1

    Close

    OrderhSMUG1

    Close

    OrderLambda Exonuclease

    Close

    OrderLambda Exonuclease Reaction Buffer

    Close

    OrderMicrococcal Nuclease

    Close

    OrderMung Bean Nuclease

    Close

    OrderNuclease BAL-31

    Close

    OrderPreCR® Repair Mix

    Close

    OrderRecAf

    Close

    OrderRecJf

    Close

    OrderT4 PDG (T4 Endonuclease V)

    Close

    OrderT5 Exonuclease

    Close

    OrderT7 Endonuclease I

    Close

    OrderT7 Exonuclease

    Close

    OrderUracil Glycosylase Inhibitor (UGI)

    Close

    OrderUracil-DNA Glycosylase (UDG)

    Phosphatases & Sulfurylases

    Close

    OrderAlkaline Phosphatase, Calf Intestinal (CIP)

    Close

    OrderAntarctic Phosphatase

    Close

    OrderAntarctic Phosphatase Reaction Buffer

    Close

    OrderApyrase

    Close

    OrderATP Sulfurylase

    Close

    Biotinylated Alkaline Phosphatase

    Close

    OrderPyrophosphatase, Inorganic (E. coli)

    Close

    OrderPyrophosphatase, Inorganic (yeast)

    Close

    OrderShrimp Alkaline Phosphatase (rSAP)

    Close

    OrderThermostable Inorganic Pyrophosphatase

    Recombinases

    Close

    OrderCre Recombinase

    Close

    OrderpLox2+ (linearized)

    ssDNA Binding Proteins

    Close

    OrderET SSB

    Close

    OrderRecA

    Close

    OrderRecAf

    Close

    OrderT4 Gene 32 Protein

    Close

    OrderTth RecA

    Synthetic Biology

    Close

    OrderBioBrick® Assembly Kit

    Close

    OrderGibson Assembly® Cloning Kit

    Close

    OrderGibson Assembly® Master Mix

    Kinases

    Close

    OrderT4 Polynucleotide Kinase

    Close

    OrderT4 Polynucleotide Kinase (3' phosphatase minus)

    Close

    OrderT4 Polynucleotide Kinase Reaction Buffer

    DNA Labeling & Chemiluminescent Detection

    Close

    NEBlot® Kit

    Close

    OrderStreptavidin

    Close

    OrderTerminal Transferase

    Close

    OrderTherminator™ DNA Polymerase

    DNA Library Preparation

    Close

    OrderNEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®

    Close

    OrderNEBNext® ChIP-Seq Library Prep Reagent Set for Illumina®

    Close

    OrderNEBNext® dA-Tailing Module

    Close

    OrderNEBNext® dA-Tailing Reaction Buffer

    Close

    OrderNEBNext® DNA Library Prep Master Mix Set for 454™

    Close

    OrderNEBNext® DNA Library Prep Master Mix Set for Illumina®

    Close

    NEBNext® DNA Library Prep Reagent Set for 454™

    Close

    OrderNEBNext® DNA Library Prep Reagent Set for Illumina®

    Close

    NEBNext® DNA Library Prep Set for SOLiD™

    Close

    OrderNEBNext® dsDNA Fragmentase®

    Close

    OrderNEBNext® End Repair Module

    Close

    OrderNEBNext® End Repair Reaction Buffer

    Close

    OrderNEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent

    Close

    OrderNEBNext® Fast DNA Library Prep Set for Ion Torrent™

    Close

    OrderNEBNext® Microbiome DNA Enrichment Kit

    Close

    OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

    Close

    OrderNEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1)

    Close

    OrderNEBNext® Quick DNA Library Prep Master Mix Set for 454™

    Close

    OrderNEBNext® Quick Ligation Module

    Close

    OrderNEBNext® Quick Ligation Reaction Buffer

    Close

    OrderNEBNext® Singleplex Oligos for Illumina®

    Close

    OrderNEBNext® Ultra™ End Repair/dA-Tailing Module

    Close

    OrderNEBNext® Ultra™ Ligation Module

    Close

    OrderNEBNext® Ultra™ DNA Library Prep Kit for Illumina®

    Cloning & Mutagenesis

    Close

    Orderdam-/dcm- Competent E. coli

    Close

    Order9°N™ DNA Ligase

    Close

    OrderCrimson LongAmp™ Taq DNA Polymerase

    Close

    OrderGibson Assembly® Cloning Kit

    Close

    OrderGibson Assembly® Master Mix

    Close

    OrderKlenow Fragment (3'→5' exo-)

    Close

    OrderLambda DNA

    Close

    OrderLambda DNA (N6-methyladenine-free)

    Close

    OrderLITMUS 28i Vector

    Close

    OrderLongAmp™ Taq DNA Polymerase

    Close

    OrderM13mp18 RF I DNA

    Close

    OrderM13mp18 Single-stranded DNA

    Close

    OrderNEB 10-beta Competent E. coli (High Efficiency)

    Close

    OrderNEB 10-beta Electrocompetent E. coli

    Close

    OrderNEB 5-alpha Competent E. coli (High Efficiency)

    Close

    OrderNEB 5-alpha Competent E. coli (Subcloning Efficiency)

    Close

    OrderNEB 5-alpha Electrocompetent E. coli

    Close

    OrderNEB 5-alpha F'Iq Competent E. coli (High Efficiency)

    Close

    OrderNEB Stable Competent E. coli (High Efficiency)

    Close

    OrderNEB Turbo Competent E. coli (High Efficiency)

    Close

    OrderNEB Turbo Electrocompetent E. coli

    Close

    OrderNEB® Essential Cloning Set

    Close

    OrderNEB® PCR Cloning Kit

    Close

    OrderpBR322 Vector

    Close

    OrderpCLuc Mini-TK 2 Vector

    Close

    OrderpCLuc-Basic 2 Vector

    Close

    OrderpGLuc Mini-TK 2 Vector

    Close

    OrderpGLuc-Basic 2 Vector

    Close

    OrderPhusion® High-Fidelity DNA Polymerase

    Close

    OrderPhusion® High-Fidelity PCR Kit

    Close

    OrderpNEB206A Linearized Vector

    Close

    OrderpSV40-GLuc Control Plasmid

    Close

    OrderpTK-CLuc Vector

    Close

    OrderpTK-GLuc Vector

    Close

    OrderpUC19 Vector

    Close

    OrderQ5® Site-Directed Mutagenesis Kit

    Close

    OrderQ5® Site-Directed Mutagenesis Kit (Without Competent Cells)

    Close

    OrderQuick Blunting™ and Quick Ligation™ Kits

    Close

    OrderQuick Blunting™ Kit

    Close

    OrderQuick Ligation™ Kit

    Close

    OrderT7 DNA Polymerase (unmodified)

    Close

    OrderUSER™ Enzyme

    Close

    OrderΦX174 RF I DNA

    Close

    OrderΦX174 RF II DNA

    Close

    OrderΦX174 Virion DNA

    Other

    Close

    Orderβ-Agarase I

    Close

    Orderβ-Agarase I Reaction Buffer

    Close

    OrderM13KO7 Helper Phage

    DNA Modifying Enzymes & Cloning Technologies Buffers & Diluents

    Close

    Orderβ-Agarase I Reaction Buffer

    Close

    OrderTaq DNA Ligase Reaction Buffer

    Close

    OrderAntarctic Phosphatase Reaction Buffer

    Close

    OrderDNase I Reaction Buffer

    Close

    OrderExonuclease I Reaction Buffer

    Close

    OrderLambda Exonuclease Reaction Buffer

    Close

    OrderT4 DNA Ligase Reaction Buffer

    Close

    OrderT4 Polynucleotide Kinase Reaction Buffer

    Close

    OrderUDG Reaction Buffer

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