A versatile E. coli strain, NEB 5-alpha is a derivative of DH5α™, and has the same genetic features of this popular cloning strain. NEB 5-alpha offers high transformation efficiencies, convenient formats and value pricing. Whether you are doing routine cloning, subcloning or looking for a high efficiency (electrocompetent) format, NEB 5-alpha is the ideal strain for you.
The NEB PCR Cloning Kit allows direct cloning from any amplification reaction, with no purification needed!
The Gibson Assembly™ Cloning Kit is a robust method for assembling 1-2 DNA fragments, with a cloning vector, and ensuring high transformation efficiencies - all in a little less than 2 hours.