Detection of specific nucleic acid sequences can be accomplished by hybridization with a labeled RNA probe. RNA probes are sequences of a variable length that are used to detect the presence of complementary nucleotide sequences in a sample. RNA probes are first labeled with radioactive nucleotides or with modified nucleotides that can be detected by fluorescence or chemiluminescence (1). RNA probes can be used for Northern blotting, RNase protection assays, Southern blotting, downstream of polymerase chain reaction (PCR), and in situ hybridization analysis.
- Cooper, Geoffery M. The Cell: A Molecular Approach. 4th ed. Washington D.C.: ASM
Press 2007. 131-2.