RNA Analysis
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  • RNA Analysis

    RNA is a polynucleotide that is typically transcribed from a DNA template. Historically, its only known function was involvement in the translation of genetic information into proteins, either through encoding the sequence of the protein (messenger RNA; mRNA), bringing amino acids to the ribosome (transfer RNA; tRNA), or by being a component of the ribosome translational machinery (ribosomal RNA; rRNA). Recently, technological advances and innovative research in the field of RNA analysis, have led to the discovery of many new classes of small and large non-coding RNAs with novel regulatory functions. These findings have helped usher in a renaissance of RNA-focused research in biology.

    RNA Analysis includes these areas of focus:

    RNA Cloning
    cDNA Synthesis & RT-PCR
    RNA Characterization & Modification
    RNA Expression Analysis
    RNA Structural Analysis
    RNA Probe Generation
    RNA Library Prep for NGS
    RNA Processing & Analysis
    RNA Detection & Isolation
    In vitro Synthesis & Processing
    Reverse Transcription

    Protocols for RNA Analysis

    RNA Reagents Overview

    Reagents for RNA Sample Preparation

    NEB offers a wide variety of reagents to facilitate front-end processing of RNA for downstream applications including next-generation sequencing, library construction and microarrays.

    RNA Polymerase Selection Chart

    NEB offers RNA polymerases that can be used for in vitro synthesis of RNA for a wide variety of downstream applications. Additional polymerases are offered for the generation of untemplated homoribopolymeric tails for reverse transcription or labeling.

    RNA Ligase Selection Chart

    NEB offers a variety of ligases for RNA research with unique specificities.

    cDNA Synthesis Selection Chart

    NEB offers several reagents for cDNA synthesis for use in applications including qPCR and qRT-PCR. For your convenience, reagents are available as kits or standalone products, depending on your needs.