Gel-based protein assays, such as SDS-PAGE and Western Blots, are used to determine the quantity and purity of a protein sample. Protein markers and ladders provide a series of graduated mass standards in a single mixture. Run alongside a sample, protein ladders provide a reference for determining the masses of all proteins within your sample.
The NEB PCR Cloning Kit allows direct cloning from any amplification reaction, with no purification needed!
The Gibson Assembly™ Cloning Kit is a robust method for assembling 1-2 DNA fragments, with a cloning vector, and ensuring high transformation efficiencies - all in a little less than 2 hours.