For use with the SOLiD™ 4 sequencing platform, mRNA libraries are prepared by construction of a cDNA library, followed by DNA library preparation steps: repair of 3’ and 5’ ends followed by ligation to an adaptor. Libraries are size selected after adaptor ligation, and amplified via polymerase chain reaction (PCR). For more information, choose the mRNA library construction workflow for SOLiD tab below.
Small RNA libraries are constructed by a different workflow, in which adaptors are ligated directly to the small RNA molecules, followed by reverse transcription, PCR amplification and size selection. In the novel NEBNext workflow, the introduction of the RT primer ahead of ligation of the 5’ adaptor prevents formation of contaminating adapter-dimers. For more information, choose the small RNA library construction workflow for SOLiD tab below.
Libraries prepared in this manner are suitable for templated bead preparation, the next step in the SOLiD sequencing workflow.
NEBNext reagents are available for each step in mRNA and small RNA library preparation workflow for the SOLiD platform. Reagents from NEB are available in a master mix format. Each of these reagents has been functionally validated by preparation of mRNA or small RNA library followed by SOLiD sequencing.
SOLiD™ is a trademark owned by Life Technologies, Inc.
NEBNext® is a registered trademark of New England Biolabs, Inc.