Library preparation of nucleic acids relies on a coordinated series of standard molecular biology reactions, and preparation of high quality libraries at high yield is a critical first step in the next generation sequencing workflow. As the number of applications in the realm of next generation sequencing increases, so does the need for reagents and protocols for library preparation.
NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA and RNA for next generation sequencing. NEBNext kits are carefully designed to improve yields and library diversity using minimal input amounts. For example, each reagent in our new NEBNext Ultra II DNA Library Prep Kit (NEB #E7645) has been reformulated, resulting in a several-fold increase in library yield with as little as 500 pg of input DNA.
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NEBNext® is a registered trademark of New England Biolabs, Inc.