• My NEB
  • Print
  • PDF
  • Glycoprotein Production in Various Expression Systems

    There is a growing interest in producing recombinant glycoproteins. In addition to enabling the researcher to produce glycoproteins for functional studies, it also importantly allows the production of therapeutic glycoproteins such as immunoglobulins for the large and growing biopharmaceutical market. While most commonly used bacterial hosts do not modify the produced protein with glycans and so are not suitable for glycoprotein expression, many eukaryotic hosts have this capability. Several dedicated systems such as mammalian, insect, yeast and plant cells have been developed to express proteins, and each has their relative advantages and disadvantages (1).

    References

    1. Betenbaugh M.J., et al (2004) Curr Opin Struct Biol. 14(5):601-6. PMID: 15465322
    • Overview of Glycobiology

      Learn about the core sequences and common modifications of N-linked and O-linked glycans in this video. Analysis of these glycans and/or peptide portions of the glycoprotein can be accomplished with the use of deglycosylation enzymes, which are explained in detail. Unlike other chemical deglycosylation methods, enzymatic treatment is much gentler and can provide complete sugar removal with no protein degradation.

      scroll to see additional videos
    • Identification and Characterization of Protein Glycosylation

      Here we illustrate the use of glycosidases for the analysis of a model glycoprotein: recombinant human chorionic gonadotropin beta (hCGβ), which carries both N-glycans and O-glycans in this video. The technique requires only simple instrumentation and typical consumables, and it can be readily adapted to the analysis of multiple glycoprotein samples.

      scroll to see additional videos
    • Elucidating the Complexity of Heparin Oligosaccharide Analysis

      Learn more about the structural elucidation of complex and diverse heparin oligosaccharides using Bacteroides Heparinase I, II and III in combination with downstream mass spec analysis.

      scroll to see additional videos

    FAQs for Glycoprotein Production in Various Expression Systems