Epigenetics

DNA Enrichment

Methyl-DNA immunoprecipitation (Me-DIP) and Methyl-CpG Binding Domain (MBD) -based capture are both effective methods to enrich methylated DNA from genomic DNA samples. However, for genome-wide methylation analysis, these two methods have differing CpG density bias. Me-DIP favors low methyl CpG density whereas MBD favors higher methyl CpG density (1). Both methods can be used upstream of microarray or sequencing analysis.

Reference

  1. Shalima S. Nair et al. (2011) Epigenetics 6:1,34-44. PMID: 20818161

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Protocols for DNA Enrichment

Overview EpiMark® Methylated DNA Enrichment Kit