DNA Modification
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  • DNA Ligation

    Ligation of DNA is a central step in many modern molecular biology workflows. DNA ligases catalyze the formation of a phosphodiester bond between the 3' hydroxyl and 5' phosphate of adjacent DNA residues. In living organisms, ligases are essential enzymes with critical roles in DNA replication and repair. In the lab, this reaction is used to join dsDNA fragments with blunt or cohesive ends to form recombinant DNA plasmids, to add bar-coded adapters to fragmented DNA during next-generation sequencing, and to join DNA probes for SNP detection using the ligase chain reaction (LCR).

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    Common Applications of Exonucleases and Endonucleases

    NEB provides a list of common applications for our exonucleases and endonucleases.

    Properties of Exonucleases and Endonucleases

    NEB supplies many nucleases; several characteristics should be considered when choosing the one best suited to your particular research needs.