DNA Modification

DNA Phosphorylation

Single- or double-stranded DNA with a 5'-hydroxyl terminus has to be phosphorylated prior to ligation as 5’ phosphate groups are required for ligation. Phosporylation of DNA ends with phosphate radioisotopes can also be used to label DNA in preparation of DNA for subsequent detection, isolation and sequencing applications. A number of polynucleotide kinases, including T4 PNK (NEB #M0201) and T4 PNK (3' phosphatase minus) (NEB #M0236), can be used to transfer the γ-phosphate of ATP to a 5’ terminus of DNA.

Featured Products

Protocols for DNA Phosphorylation

Common Applications of Exonucleases and Endonucleases

NEB provides a list of common applications for our exonucleases and endonucleases.

Properties of Exonucleases and Endonucleases

NEB supplies many nucleases; several characteristics should be considered when choosing the one best suited to your particular research needs.