DNA Amplification
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  • High-Fidelity PCR

    The fidelity of a polymerase refers to its ability to insert the correct base during PCR. Conversely, the rate of misincorporation is known as a polymerase’s error rate. High-fidelity PCR, utilizes a DNA polymerase with a low error rate and results in a high degree of accuracy in the replication of the DNA of interest. NEB scientists were the first to identify and commercialize a high-fidelity DNA polymerase suitable for PCR, namely VentR® 

    DNA Polymerase.Since its discovery, several other high fidelity polymerases have been discovered and engineered – all with differing processivity, speed and levels of accuracy..

    When do you need a high-fidelity DNA polymerase?

    1. How Can I Improve Amplification of GC-rich Regions

      The secondary structures that form within GC-rich regions can make efficient DNA amplification difficult. Nicole’s suggestions can help!

    2. DNA Replication with a Proofreading Polymerase

      Learn how proofreading polymerases recognize and correct mismatched bases.

    3. How to Amplify GC-rich DNA

      Looking for tips on dealing with GC-bias in DNA amplification? NEB scientists have the expertise you need!

    4. Choose the Right DNA Polymerase for PCR

      Make sure you're using the optimal polymerase for your DNA amplifications. Get tips on choosing the right DNA Polymerase for your application.

    5. Important Tips for Q5® Polymerase

      Here are some quick tips for getting the most out of NEB's Q5® High-Fidelity DNA Polymerase.

    6. Why Choose Q5® High-fidelity Polymerase?

      Not sure why Q5® is your best choice for high-fidelity amplification of GC-rich targets? NEB's scientists will show you why we call Q5 an "ultra-high fidelity polymerase".

    FAQs for High-Fidelity PCR

    Protocols for High-Fidelity PCR