The Polymerase Chain Reaction (PCR) is a well-known approach to amplify a specific DNA sequence. PCR involves the reiterative cycling of a reaction cocktail between different temperatures to achieve amplification. As routine as PCR is in the molecular biology and molecular diagnostic laboratory, there are other methods of sequence-specific DNA amplification. These alternative approaches often do not require changing the reaction temperature and are, therefore, often referred to as sequence-specific isothermal amplification protocols. Isothermal amplification protocols are varied and have varied advantages. However, some common advantages are that isothermal techniques are extremely fast and they do not require thermocyclers.
Four examples of sequence-specific isothermal DNA amplification technologies include: