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  • DNA Probe Generation

    DNA probes can be generated to identify or isolate specific DNA sequences of interest by labeling (via radio- or fluorescently-labeled nucleotides) or affinity-tagging DNA that is complementary to the target DNA. The DNA probe is allowed to hybridize with the target sequence, confirming the location or presence of the target sequence. Affinity tags may allow isolation of the tagged DNA whereas fluorescence tags (as in fluorescence in situ hybridization) may allow the position of the sequence of interest to be identified along a chromosome and copy number to be determined.

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