Cloning & Mapping Products

New England Biolabs was built on the promise of providing highly-pure molecular biology reagents of the highest quality. For cloning and mapping, NEB offers a wide range of products, including restriction endonucleases, polymerases for PCR, modifying enzymes, expression vectors, competent cells and cloning kits, including the Gibson Assembly® Cloning Kit.

Gibson Assembly® is a trademark of Synthetic Genomics, Inc.

Cloning & Mapping

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OrderCas9 Nuclease, S. pyogenes

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OrderEnGen® Cas9 NLS, S. pyogenes

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OrderNEB® PCR Cloning Kit

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OrderNEB® PCR Cloning Kit (Without Competent Cells)

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OrderNEBuilder® HiFi DNA Assembly Bundle for Large Fragments

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OrderNEBuilder® HiFi DNA Assembly Master Mix

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OrderNEBuilder® HiFi DNA Assembly Cloning Kit

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OrderNuclease-free Water

Transformation

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Orderdam-/dcm- Competent E. coli

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OrderNEB® 10-beta Competent E. coli (High Efficiency)

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OrderNEB® 10-beta Electrocompetent E. coli

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OrderNEB® 5-alpha Competent E. coli (High Efficiency)

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OrderNEB® 5-alpha Competent E. coli (Subcloning Efficiency)

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OrderNEB® 5-alpha Electrocompetent E. coli

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OrderNEB® 5-alpha F'Iq Competent E. coli (High Efficiency)

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OrderNEB® Stable Competent E. coli (High Efficiency)

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OrderNEB® Turbo Competent E. coli (High Efficiency)

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OrderNEB® Turbo Electrocompetent E. coli

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OrderSOC Outgrowth Medium

Site Directed Mutagenesis

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OrderDpnI

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OrderNEB® 10-beta Competent E. coli (High Efficiency)

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OrderQ5® Hot Start High-Fidelity 2X Master Mix

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OrderQ5® Site-Directed Mutagenesis Kit

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OrderQ5® Site-Directed Mutagenesis Kit (Without Competent Cells)

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OrderSOC Outgrowth Medium

DNA Analysis

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Orderλ DNA-BstEII Digest

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Orderλ DNA-HindIII Digest

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Orderλ DNA-Mono Cut Mix

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Order1 kb DNA Ladder

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Order100 bp DNA Ladder

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Order2-Log DNA Ladder (0.1-10.0 kb)

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Order50 bp DNA Ladder

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OrderFast DNA Ladder

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OrderLambda PFG Ladder

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OrderLow Molecular Weight DNA Ladder

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OrderpBR322 DNA-BstNI Digest

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OrderpBR322 DNA-MspI Digest

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OrderPCR Marker

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OrderQuick-Load® Purple 50 bp DNA Ladder

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OrderQuick-Load® Purple Low Molecular Weight DNA Ladder

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OrderQuick-Load® 1 kb DNA Ladder

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OrderQuick-Load® 1 kb Extend DNA Ladder

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OrderQuick-Load® 100 bp DNA Ladder

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OrderQuick-Load® 2-Log DNA Ladder(0.1-10.0 kb)

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OrderQuick-Load® Purple 1 kb DNA Ladder

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OrderQuick-Load® Purple 100 bp DNA Ladder

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OrderQuick-Load® Purple 2-Log DNA Ladder (0.1 - 10.0 kb)

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OrderSupercoiled DNA Ladder

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OrderTriDye™ 1 kb DNA Ladder

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OrderTriDye™ 100 bp DNA Ladder

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OrderTriDye™ 2-Log DNA Ladder (0.1 - 10.0 kb)

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OrderΦX174 DNA-Hae III Digest

DNA Isolation

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Orderβ-Agarase I

Synthetic Biology

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OrderNEB® Golden Gate Assembly Mix

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OrderUSER™ Enzyme

Gibson Assembly®

DNA Ligation

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OrderHiFi Taq DNA Ligase

DNA End Treatment

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OrderAlkaline Phosphatase, Calf Intestinal (CIP)

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OrderAntarctic Phosphatase

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OrderAntarctic Phosphatase Reaction Buffer

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Biotinylated Alkaline Phosphatase

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OrderDNA Polymerase I, Large (Klenow) Fragment

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OrderMung Bean Nuclease

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OrderQuick Blunting™ and Quick Ligation™ Kits

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OrderQuick Blunting™ Kit

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OrderQuick Dephosphorylation Kit

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OrderShrimp Alkaline Phosphatase (rSAP)

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OrderT4 DNA Ligase Reaction Buffer

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OrderT4 DNA Polymerase

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OrderT4 Polynucleotide Kinase

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OrderT4 Polynucleotide Kinase (3' phosphatase minus)

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OrderT4 Polynucleotide Kinase Reaction Buffer

DNA Digestion

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OrderAatII

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OrderAbaSI

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OrderAcc65I

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OrderAccI

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OrderAciI

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OrderAclI

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OrderAcuI

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OrderAfeI

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OrderAflII

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OrderAflIII

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OrderAgeI

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OrderAgeI-HF®

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OrderAhdI

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OrderAleI

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OrderAluI

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OrderAlwI

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OrderAlwNI

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OrderApaI

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OrderApaLI

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OrderApeKI

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OrderApoI

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OrderApoI-HF®

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OrderAscI

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OrderAseI

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OrderAsiSI

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OrderAvaI

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OrderAvaII

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OrderAvrII

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OrderBaeGI

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OrderBaeI

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OrderBamHI

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OrderBamHI-HF®

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OrderBanI

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OrderBanII

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OrderBbsI

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OrderBbsI-HF®

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OrderBbvCI

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OrderBbvI

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OrderBccI

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OrderBceAI

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OrderBcgI

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OrderBciVI

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OrderBclI

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OrderBcoDI

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OrderBfaI

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OrderBfuAI

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OrderBfuCI

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OrderBglI

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OrderBglII

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OrderBlpI

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OrderBmgBI

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OrderBmrI

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OrderBmtI

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OrderBmtI-HF®

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OrderBpmI

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OrderBpu10I

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OrderBpuEI

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OrderBSA, Molecular Biology Grade

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OrderBsaAI

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OrderBsaBI

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OrderBsaHI

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OrderBsaI

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OrderBsaI-HF®

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OrderBsaJI

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OrderBsaWI

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OrderBsaXI

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OrderBseRI

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OrderBseYI

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OrderBsgI

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OrderBsiEI

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OrderBsiHKAI

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OrderBsiWI

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OrderBslI

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OrderBsmAI

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OrderBsmBI

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OrderBsmFI

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OrderBsmI

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OrderBsoBI

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OrderBsp1286I

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OrderBspCNI

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OrderBspDI

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OrderBspEI

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OrderBspHI

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OrderBspMI

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OrderBspQI

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OrderBsrBI

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OrderBsrDI

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OrderBsrFαI

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OrderBsrGI

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OrderBsrI

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OrderBssHII

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BssKI

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OrderBssSαI

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OrderBstAPI

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OrderBstBI

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OrderBstEII

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OrderBstEII-HF®

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OrderBstNI

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OrderBstUI

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OrderBstYI

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OrderBstZ17I

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OrderBsu36I

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OrderBtgI

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OrderBtgZI

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OrderBtsαI

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OrderBtsCI

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OrderBtsIMutI

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OrderCac8I

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OrderClaI

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OrderCspCI

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OrderCutSmart® Buffer

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OrderCviAII

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OrderCviKI-1

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OrderCviQI

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OrderDdeI

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OrderDiluent A

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OrderDiluent B

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OrderDiluent C

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OrderDpnI

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OrderDpnII

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OrderDraI

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OrderDraIII-HF®

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OrderDrdI

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OrderEaeI

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OrderEagI

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OrderEagI-HF®

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OrderEarI

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OrderEciI

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OrderEco53kI

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OrderEcoNI

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OrderEcoO109I

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OrderEcoP15I

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OrderEcoRI

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OrderEcoRI-HF®

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OrderEcoRV

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OrderEcoRV-HF®

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OrderFatI

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OrderFauI

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OrderFnu4HI

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OrderFokI

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OrderFseI

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OrderFspEI

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OrderFspI

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OrderHaeII

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OrderHaeIII

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OrderHgaI

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OrderHhaI

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OrderHincII

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OrderHindIII

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OrderHindIII-HF®

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OrderHinfI

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OrderHinP1I

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OrderHpaI

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OrderHpaII

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OrderHphI

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OrderHpy166II

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OrderHpy188I

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OrderHpy188III

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OrderHpy99I

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OrderHpyCH4III

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OrderHpyCH4IV

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OrderHpyCH4V

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OrderI-CeuI

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OrderI-SceI

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OrderKasI

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OrderKpnI

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OrderKpnI-HF®

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OrderLpnPI

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OrderMboI

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OrderMboII

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OrderMcrBC

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OrderMfeI

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OrderMfeI-HF®

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OrderMluCI

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OrderMluI

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OrderMluI-HF®

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OrderMlyI

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OrderMmeI

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OrderMnlI

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OrderMscI

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OrderMseI

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OrderMslI

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OrderMspA1I

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OrderMspI

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OrderMspJI

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OrderMwoI

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OrderNaeI

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OrderNarI

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OrderNciI

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OrderNcoI

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OrderNcoI-HF®

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OrderNdeI

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OrderNEBuffer 1

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OrderNEBuffer 1.1

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OrderNEBuffer 2

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OrderNEBuffer 2.1

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OrderNEBuffer 3

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OrderNEBuffer 3.1

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OrderNEBuffer 4

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OrderNEBuffer EcoRI

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OrderNEBuffer Set 1.1, 2.1, 3.1 and CutSmart

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OrderNgoMIV

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OrderNheI

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OrderNheI-HF®

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OrderNlaIII

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OrderNlaIV

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OrderNmeAIII

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OrderNotI

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OrderNotI-HF®

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OrderNruI

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OrderNruI-HF®

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OrderNsiI

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OrderNsiI-HF®

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OrderNspI

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OrderPacI

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OrderPaeR7I

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OrderPciI

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OrderPflFI

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OrderPflMI

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OrderPI-PspI

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OrderPI-SceI

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OrderPleI

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OrderPmeI

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OrderPmlI

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OrderPpuMI

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OrderPshAI

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OrderPsiI

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OrderPspGI

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OrderPspOMI

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OrderPspXI

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OrderPstI-HF®

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OrderPvuI

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OrderPvuI-HF®

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OrderPvuII

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OrderPvuII-HF®

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OrderRsaI

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OrderRsrII

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OrderSacI

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OrderSacI-HF®

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OrderSacII

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OrderS-adenosylmethionine (SAM)

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OrderSalI

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OrderSalI-HF®

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OrderSapI

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OrderSau3AI

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OrderSau96I

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OrderSbfI

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OrderSbfI-HF®

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OrderScaI-HF®

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OrderScrFI

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OrderSexAI

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OrderSfaNI

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OrderSfcI

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OrderSfiI

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OrderSfoI

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OrderSgrAI

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OrderSmaI

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OrderSmlI

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OrderSnaBI

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OrderSpeI

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SpeI RE-Mix®

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OrderSpeI-HF®

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OrderSphI

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OrderSphI-HF®

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OrderSrfI

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OrderSspI

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OrderSspI-HF®

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OrderStuI

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OrderStyD4I

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OrderStyI

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OrderStyI-HF®

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OrderSwaI

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OrderTaqαI

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OrderTfiI

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TliI

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OrderTseI

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OrderTsp45I

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Tsp509I

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OrderTspMI

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OrderTspRI

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OrderTth111I

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OrderXbaI

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OrderXcmI

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OrderXhoI

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OrderXmaI

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OrderXmnI

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OrderZraI

Transformation

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Orderdam-/dcm- Competent E. coli

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OrderNEB® 10-beta Competent E. coli (High Efficiency)

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OrderNEB® 10-beta Electrocompetent E. coli

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OrderNEB® 5-alpha Competent E. coli (High Efficiency)

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OrderNEB® 5-alpha Competent E. coli (Subcloning Efficiency)

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OrderNEB® 5-alpha Electrocompetent E. coli

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OrderNEB® 5-alpha F'Iq Competent E. coli (High Efficiency)

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OrderNEB® Stable Competent E. coli (High Efficiency)

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OrderNEB® Turbo Competent E. coli (High Efficiency)

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OrderNEB® Turbo Electrocompetent E. coli

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OrderSOC Outgrowth Medium

Site Directed Mutagenesis

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OrderDpnI

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OrderNEB® 10-beta Competent E. coli (High Efficiency)

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OrderQ5® Hot Start High-Fidelity 2X Master Mix

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OrderQ5® Site-Directed Mutagenesis Kit

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OrderQ5® Site-Directed Mutagenesis Kit (Without Competent Cells)

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OrderSOC Outgrowth Medium

DNA Analysis

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Orderλ DNA-BstEII Digest

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Orderλ DNA-HindIII Digest

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Orderλ DNA-Mono Cut Mix

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Order1 kb DNA Ladder

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Order100 bp DNA Ladder

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Order2-Log DNA Ladder (0.1-10.0 kb)

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Order50 bp DNA Ladder

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OrderFast DNA Ladder

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OrderLambda PFG Ladder

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OrderLow Molecular Weight DNA Ladder

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OrderpBR322 DNA-BstNI Digest

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OrderpBR322 DNA-MspI Digest

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OrderPCR Marker

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OrderQuick-Load® Purple 50 bp DNA Ladder

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OrderQuick-Load® Purple Low Molecular Weight DNA Ladder

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OrderQuick-Load® 1 kb DNA Ladder

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OrderQuick-Load® 1 kb Extend DNA Ladder

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OrderQuick-Load® 100 bp DNA Ladder

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OrderQuick-Load® 2-Log DNA Ladder(0.1-10.0 kb)

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OrderQuick-Load® Purple 1 kb DNA Ladder

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OrderQuick-Load® Purple 100 bp DNA Ladder

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OrderQuick-Load® Purple 2-Log DNA Ladder (0.1 - 10.0 kb)

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OrderSupercoiled DNA Ladder

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OrderTriDye™ 1 kb DNA Ladder

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OrderTriDye™ 100 bp DNA Ladder

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OrderTriDye™ 2-Log DNA Ladder (0.1 - 10.0 kb)

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OrderΦX174 DNA-Hae III Digest

DNA Isolation

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Orderβ-Agarase I

Synthetic Biology

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OrderNEB® Golden Gate Assembly Mix

Close

OrderUSER™ Enzyme

Gibson Assembly®

DNA Ligation

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OrderHiFi Taq DNA Ligase

DNA End Treatment

DNA Phosphorylation

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OrderQuick Blunting™ and Quick Ligation™ Kits

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OrderQuick Blunting™ Kit

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OrderT4 DNA Ligase Reaction Buffer

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OrderT4 Polynucleotide Kinase

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OrderT4 Polynucleotide Kinase (3' phosphatase minus)

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OrderT4 Polynucleotide Kinase Reaction Buffer

DNA Blunting

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OrderDNA Polymerase I, Large (Klenow) Fragment

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OrderMung Bean Nuclease

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OrderQuick Blunting™ and Quick Ligation™ Kits

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OrderQuick Blunting™ Kit

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OrderT4 DNA Polymerase

DNA Dephosphorylation

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OrderAlkaline Phosphatase, Calf Intestinal (CIP)

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OrderAntarctic Phosphatase

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OrderAntarctic Phosphatase Reaction Buffer

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Biotinylated Alkaline Phosphatase

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OrderQuick Dephosphorylation Kit

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OrderShrimp Alkaline Phosphatase (rSAP)

DNA Digestion

Restriction Enzyme Digestion

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OrderAatII

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OrderAbaSI

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OrderAcc65I

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OrderAccI

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OrderAciI

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OrderAclI

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OrderAcuI

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OrderAfeI

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OrderAflII

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OrderAflIII

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OrderAgeI

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OrderAgeI-HF®

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OrderAhdI

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OrderAleI

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OrderAluI

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OrderAlwI

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OrderAlwNI

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OrderApaI

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OrderApaLI

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OrderApeKI

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OrderApoI

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OrderApoI-HF®

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OrderAscI

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OrderAseI

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OrderAsiSI

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OrderAvaI

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OrderAvaII

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OrderAvrII

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OrderBaeGI

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OrderBaeI

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OrderBamHI

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OrderBamHI-HF®

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OrderBanI

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OrderBanII

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OrderBbsI

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OrderBbsI-HF®

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OrderBbvCI

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OrderBbvI

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OrderBccI

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OrderBceAI

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OrderBcgI

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OrderBciVI

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OrderBclI

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OrderBcoDI

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OrderBfaI

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OrderBfuAI

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OrderBfuCI

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OrderBglI

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OrderBglII

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OrderBlpI

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OrderBmgBI

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OrderBmrI

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OrderBmtI

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OrderBmtI-HF®

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OrderBpmI

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OrderBpu10I

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OrderBpuEI

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OrderBSA, Molecular Biology Grade

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OrderBsaAI

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OrderBsaBI

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OrderBsaHI

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OrderBsaI

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OrderBsaI-HF®

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OrderBsaJI

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OrderBsaWI

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OrderBsaXI

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OrderBseRI

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OrderBseYI

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OrderBsgI

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OrderBsiEI

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OrderBsiHKAI

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OrderBsiWI

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OrderBslI

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OrderBsmAI

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OrderBsmBI

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OrderBsmFI

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OrderBsmI

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OrderBsoBI

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OrderBsp1286I

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OrderBspCNI

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OrderBspDI

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OrderBspEI

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OrderBspHI

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OrderBspMI

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OrderBspQI

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OrderBsrBI

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OrderBsrDI

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OrderBsrFαI

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OrderBsrGI

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OrderBsrI

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OrderBssHII

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BssKI

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OrderBssSαI

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OrderBstAPI

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OrderBstBI

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OrderBstEII

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OrderBstEII-HF®

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OrderBstNI

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OrderBstUI

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OrderBstYI

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OrderBstZ17I

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OrderBsu36I

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OrderBtgI

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OrderBtgZI

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OrderBtsαI

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OrderBtsCI

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OrderBtsIMutI

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OrderCac8I

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OrderClaI

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OrderCspCI

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OrderCutSmart® Buffer

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OrderCviAII

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OrderCviKI-1

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OrderCviQI

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OrderDdeI

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OrderDiluent A

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OrderDiluent B

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OrderDiluent C

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OrderDpnI

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OrderDpnII

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OrderDraI

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OrderDraIII-HF®

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OrderDrdI

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OrderEaeI

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OrderEagI

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OrderEagI-HF®

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OrderEarI

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OrderEciI

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OrderEco53kI

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OrderEcoNI

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OrderEcoO109I

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OrderEcoP15I

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OrderEcoRI

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OrderEcoRI-HF®

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OrderEcoRV

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OrderEcoRV-HF®

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OrderFatI

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OrderFauI

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OrderFnu4HI

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OrderFokI

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OrderFseI

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OrderFspEI

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OrderFspI

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OrderHaeII

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OrderHaeIII

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OrderHgaI

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OrderHhaI

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OrderHincII

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OrderHindIII

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OrderHindIII-HF®

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OrderHinfI

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OrderHinP1I

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OrderHpaI

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OrderHpaII

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OrderHphI

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OrderHpy166II

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OrderHpy188I

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OrderHpy188III

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OrderHpy99I

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OrderHpyCH4III

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OrderHpyCH4IV

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OrderHpyCH4V

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OrderI-CeuI

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OrderI-SceI

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OrderKasI

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OrderKpnI

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OrderKpnI-HF®

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OrderLpnPI

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OrderMboI

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OrderMboII

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OrderMcrBC

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OrderMfeI

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OrderMfeI-HF®

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OrderMluCI

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OrderMluI

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OrderMluI-HF®

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OrderMlyI

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OrderMmeI

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OrderMnlI

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OrderMscI

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OrderMseI

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OrderMslI

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OrderMspA1I

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OrderMspI

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OrderMspJI

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OrderMwoI

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OrderNaeI

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OrderNarI

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OrderNciI

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OrderNcoI

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OrderNcoI-HF®

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OrderNdeI

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OrderNEBuffer 1

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OrderNEBuffer 1.1

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OrderNEBuffer 2

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OrderNEBuffer 2.1

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OrderNEBuffer 3

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OrderNEBuffer 3.1

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OrderNEBuffer 4

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OrderNEBuffer EcoRI

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OrderNEBuffer Set 1.1, 2.1, 3.1 and CutSmart

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OrderNgoMIV

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OrderNheI

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OrderNheI-HF®

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OrderNlaIII

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OrderNlaIV

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OrderNmeAIII

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OrderNotI

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OrderNotI-HF®

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OrderNruI

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OrderNruI-HF®

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OrderNsiI

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OrderNsiI-HF®

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OrderNspI

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OrderPacI

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OrderPaeR7I

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OrderPciI

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OrderPflFI

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OrderPflMI

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OrderPI-PspI

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OrderPI-SceI

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OrderPleI

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OrderPmeI

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OrderPmlI

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OrderPpuMI

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OrderPshAI

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OrderPsiI

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OrderPspGI

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OrderPspOMI

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OrderPspXI

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OrderPstI-HF®

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OrderPvuI

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OrderPvuI-HF®

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OrderPvuII

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OrderPvuII-HF®

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OrderRsaI

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OrderRsrII

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OrderSacI

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OrderSacI-HF®

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OrderSacII

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OrderS-adenosylmethionine (SAM)

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OrderSalI

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OrderSalI-HF®

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OrderSapI

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OrderSau3AI

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OrderSau96I

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OrderSbfI

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OrderSbfI-HF®

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OrderScaI-HF®

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OrderScrFI

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OrderSexAI

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OrderSfaNI

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OrderSfcI

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OrderSfiI

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OrderSfoI

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OrderSgrAI

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OrderSmaI

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OrderSmlI

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OrderSnaBI

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OrderSpeI

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SpeI RE-Mix®

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OrderSpeI-HF®

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OrderSphI

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OrderSphI-HF®

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OrderSrfI

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OrderSspI

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OrderSspI-HF®

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OrderStuI

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OrderStyD4I

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OrderStyI

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OrderStyI-HF®

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OrderSwaI

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OrderTaqαI

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OrderTfiI

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TliI

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OrderTseI

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OrderTsp45I

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Tsp509I

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OrderTspMI

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OrderTspRI

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OrderTth111I

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OrderXbaI

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OrderXcmI

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OrderXhoI

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OrderXmaI

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OrderXmnI

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OrderZraI

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