Type II restriction enzymes are most commonly used for molecular biology applications, as they recognize stereotypical sequences and produce a predictable cleavage pattern. Learn more about how Type II REs work.
Type I restriction enzymes are a group of endonucleases that recognize a bipartite sequence, but do not produce a predictable cleavage pattern. Learn more about how Type I REs work.
Type III restriction enzymes are a group of endonucleases that recognize a non-pallindromic sequence, comprising two inversely oriented sites. Learn more about these poorly understood enzymes.
Let one of NEB's restriction enzyme experts help you improve your technique and avoid common mistakes in digest setup.
Not getting the cleavage you expected? Let an NEB scientist help you troubleshoot your reaction.
Are you finding unexpected bands in your digestion reaction? Here are some tips to help you determine the cause.
Learn what Star Activity is, why it is detrimental to accurate restriction enzyme digestion, and how NEB's HF™ enzymes are engineered to avoid it.
RE-Mix® Restriction Enzyme Master Mixes offer simplified reaction setup. Learn more about digesting DNA with RE-Mix.
Double digests are now easier than ever! Learn how to set up your next double digest with RE-Mix®.
NEB has engineered HF™ enzymes to eliminate star activity. Learn how, and what this means for your digests.
Double digestions can save you time, and this video can offer tips for how to achieve the best results, no matter which of NEB's restriction enzymes you're using.
When cutting close to the end of a DNA molecule, make sure you know how many bases to add to the ends of your PCR primers.
Learn more about what causes this common problem, and how NEB's enzymes are QC'd to avoid DNA smearing.
Restriction enzymes are an integral part of the cloning workflow, for generating compatible ends on fragments and vectors. This animation discusses three guidelines for determining which restriction enzymes to use in your cloning experiment.